ArticleThe Journal of cell biology2000
Dynein is a transient kinetochore component whose binding is regulated by microtubule attachment, not tension.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 115 citations in OpenAlex.
- Functional Interaction of SKA and NDC80 Complexes at Kinetochores Promoting Anaphase Onset in Mitosis.bioRxiv : the preprint server for biology · 2026Article
- Kinetochore-microtubule error correction for biorientation: lessons from yeast.Biochemical Society transactions · 2024Review
- The role of kinetochore dynein in checkpoint silencing is restricted to disassembly of the corona.Molecular biology of the cell · 2023Article
- Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.Molecular biology of the cell · 2023Article
- A moderate static magnetic field promotes C. elegans longevity through cytochrome P450s.Scientific reports · 2022Article
- Swap and stop - Kinetochores play error correction with microtubules: Mechanisms of kinetochore-microtubule error correction: Mechanisms of kinetochore-microtubule error correction.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Review
- The decrease of intraflagellar transport impairs sensory perception and metabolism in ageing.Nature communications · 2021Article
- Asymmetric Histone Inheritance in Asymmetrically Dividing Stem Cells.Trends in genetics : TIG · 2020Review
- A microRNA switch controls dietary restriction-induced longevity through Wnt signaling.EMBO reports · 2019Article
- Cdk1 phosphorylation of the dynein adapter Nde1 controls cargo binding from G2 to anaphase.The Journal of cell biology · 2018Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.Nature communications · 2016Article
- Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing.The Journal of cell biology · 2015Article
- A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores.The Journal of cell biology · 2015Article
- Function and regulation of dynein in mitotic chromosome segregation.Chromosoma · 2014Review
- The current view for the silencing of the spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2014Review
- Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores.The Journal of cell biology · 2013Article
- New insights into the mechanism for chromosome alignment in metaphase.International review of cell and molecular biology · 2013Review
- A steroid receptor-microRNA switch regulates life span in response to signals from the gonad.Science (New York, N.Y.) · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Cytoplasmic dynein is the only known kinetochore protein capable of driving chromosome movement toward spindle poles. In grasshopper spermatocytes, dynein immunofluorescence staining is bright at prometaphase kinetochores and dimmer at metaphase kinetochores. We have determined that these differences in staining intensity reflect differences in amounts of dynein associated with the kinetochore. Metaphase kinetochores regain bright dynein staining if they are detached from spindle microtubules by micromanipulation and kept detached for 10 min. We show that this increase in dynein staining is not caused by the retraction or unmasking of dynein upon detachment. Thus, dynein genuinely is a transient component of spermatocyte kinetochores. We further show that microtubule attachment, not tension, regulates dynein localization at kinetochores. Dynein binding is extremely sensitive to the presence of microtubules: fewer than half the normal number of kinetochore microtubules leads to the loss of most kinetochoric dynein. As a result, the bulk of the dynein leaves the kinetochore very early in mitosis, soon after the kinetochores begin to attach to microtubules. The possible functions of this dynein fraction are therefore limited to the initial attachment and movement of chromosomes and/or to a role in the mitotic checkpoint.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.