ArticleMolecular and cellular biology2000
DNA integration by Ty integrase in yku70 mutant Saccharomyces cerevisiae cells.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Article
- Host proteins interacting with the Moloney murine leukemia virus integrase: multiple transcriptional regulators and chromatin binding factors.Retrovirology · 2008Article
- Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.Nucleic acids research · 2002Article
- Ku and the Stability of the Genome.Journal of biomedicine & biotechnology · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In the present work we examined nonhomologous integration of plasmid DNA in a yku70 mutant. Ten of 14 plasmids integrated as composite elements, including Ty sequences probably originating from erroneous strand-switching and/or priming events. Three additional plasmids integrated via Ty integrase without cointegrating Ty sequences, as inferred from 5-bp target site duplication and integration site preferences. Ty integrase-mediated integration of non-Ty DNA has never been observed in wild-type cells, although purified integrase is capable of using non-Ty DNA as a substrate in vitro. Hence our data implicate yKu70 as the cellular function preventing integrase from accepting non-Ty DNA as a substrate.
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