ArticleMolecular and cellular biology2000
Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1).
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- CIP/KIP and INK4 families as hostages of oncogenic signaling.Cell division · 2024Review
- CDK4: a master regulator of the cell cycle and its role in cancer.Genes & cancer · 2022Review
- Proliferation of hippocampal progenitors relies on p27-dependent regulation of Cdk6 kinase activity.Cellular and molecular life sciences : CMLS · 2018Article
- An Elk transcription factor is required for Runx-dependent survival signaling in the sea urchin embryo.Developmental biology · 2016Article
- The non-canonical functions of p27(Kip1) in normal and tumor biology.Cell cycle (Georgetown, Tex.) · 2016Review
- Evidence for a CDK4-dependent checkpoint in a conditional model of cellular senescence.Cell cycle (Georgetown, Tex.) · 2015Article
- Genetic characterization of the role of the Cip/Kip family of proteins as cyclin-dependent kinase inhibitors and assembly factors.Molecular and cellular biology · 2014Article
- The CDK4/CDK6 inhibitor PD0332991 paradoxically stabilizes activated cyclin D3-CDK4/6 complexes.Cell cycle (Georgetown, Tex.) · 2014Article
- Vitamin E δ-tocotrienol induces p27(Kip1)-dependent cell-cycle arrest in pancreatic cancer cells via an E2F-1-dependent mechanism.PloS one · 2013Article
- CDK4: A Key Player in the Cell Cycle, Development, and Cancer.Genes & cancer · 2012Article
- A p27Kip1 mutant that does not inhibit CDK activity promotes centrosome amplification and micronucleation.Oncogene · 2012Article
- Blockade of protein geranylgeranylation inhibits Cdk2-dependent p27Kip1 phosphorylation on Thr187 and accumulates p27Kip1 in the nucleus: implications for breast cancer therapy.Molecular and cellular biology · 2009Article
- Phosphorylation of p27Kip1 regulates assembly and activation of cyclin D1-Cdk4.Molecular and cellular biology · 2008Article
- Increased expression of CDK11p58 and cyclin D3 following spinal cord injury in rats.Molecular and cellular biochemistry · 2008Article
- Regulation of CDK4.Cell division · 2006Article
- Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors".Molecular and cellular biology · 2006Article
- Novel functions of plant cyclin-dependent kinase inhibitors, ICK1/KRP1, can act non-cell-autonomously and inhibit entry into mitosis.The Plant cell · 2005Article
- Article
- Biochemical and cellular mechanisms of mammalian CDK inhibitors: a few unresolved issues.Oncogene · 2005Review
- A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Our studies examined the effects of p27(kip1) and p21(cip1) on the assembly and activity of cyclin D3-cdk4 complexes and determined the composition of the cyclin D3 pool in cells containing and lacking these cyclin-dependent kinase inhibitors. We found that catalytically active cyclin D3-cdk4 complexes were present in fibroblasts derived from p27(kip1)-p21(cip1)-null mice and that immunodepletion of extracts of wild-type cells with antibody to p27(kip1) and/or p21(cip1) removed cyclin D3 protein but not cyclin D3-associated activity. Similar results were observed in experiments assaying cyclin D1-cdk4 activity. Data obtained using mixed cell extracts demonstrated that p27(kip1) interacted with cyclin D3-cdk4 complexes in vitro and that this interaction was paralleled by a loss of cyclin D3-cdk4 activity. In p27(kip1)-p21(cip1)-deficient cells, the cyclin D3 pool consisted primarily of cyclin D3 monomers, whereas in wild-type cells, the majority of cyclin D3 molecules were complexed to cdk4 and either p27(kip1) or p21(cip1) or were monomeric. We conclude that neither p27(kip1) nor p21(cip1) is required for the formation of cyclin D3-cdk4 complexes and that cyclin D3-cdk4 complexes containing p27(kip1) or p21(cip1) are inactive. We suggest that only a minor portion of the total cyclin D3 pool accounts for all of the cyclin D3-cdk4 activity in the cell regardless of whether the cell contains p27(kip1) and p21(cip1).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.