ArticleMolecular and cellular biology2000
In vivo requirement of activator-specific binding targets of mediator.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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Who cites it
67 citing papers in PubMed.
- UBIQUITOUS FUNCTIONAL SYNERGY PARTIALLY EXPLAINS WHY MOST TRANSCRIPTION FACTOR BINDING IS NON-FUNCTIONAL.bioRxiv : the preprint server for biology · 2026Article
- Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism.Molecular cell · 2022Article
- The SAGA complex regulates early steps in transcription via its deubiquitylase module subunit USP22.The EMBO journal · 2021Article
- CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.Cell death and differentiation · 2021Article
- Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II.PLoS genetics · 2020Article
- The Mediator Subunit, Med23 Is Required for Embryonic Survival and Regulation of Canonical WNT Signaling During Cranial Ganglia Development.Frontiers in physiology · 2020Article
- Transcriptome Analysis of FourG3 (Bethesda, Md.) · 2018Article
- ETV4 and AP1 Transcription Factors Form Multivalent Interactions with three Sites on the MED25 Activator-Interacting Domain.Journal of molecular biology · 2017Article
- Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo.Molecular cell · 2016Article
- Mediator Undergoes a Compositional Change during Transcriptional Activation.Molecular cell · 2016Article
- Article
- Proteomic analysis of the herpes simplex virus 1 virion protein 16 transactivator protein in infected cells.Proteomics · 2015Article
- Article
- A functional portrait of Med7 and the mediator complex in Candida albicans.PLoS genetics · 2014Article
- Article
- The Mediator complex subunit MED25 is targeted by the N-terminal transactivation domain of the PEA3 group members.Nucleic acids research · 2013Article
- Mediator phosphorylation prevents stress response transcription during non-stress conditions.The Journal of biological chemistry · 2012Article
- Analysis of Gal4-directed transcription activation using Tra1 mutants selectively defective for interaction with Gal4.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.The EMBO journal · 2012Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There has been no unequivocal demonstration that the activator binding targets identified in vitro play a key role in transcriptional activation in vivo. To examine whether activator-Mediator interactions are required for gene transcription under physiological conditions, we performed functional analyses with Mediator components that interact specifically with natural yeast activators. Different activators interact with Mediator via distinct binding targets. Deletion of a distinct activator binding region of Mediator completely compromised gene activation in vivo by some, but not all, transcriptional activators. These demonstrate that the activator-specific targets in Mediator are essential for transcriptional activation in living cells, but their requirement was affected by the nature of the activator-DNA interaction and the existence of a postrecruitment activation process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.