ArticleMolecular and cellular biology2000
Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
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- Linking Cellular Morphogenesis with Antifungal Treatment and Susceptibility inJournal of fungi (Basel, Switzerland) · 2019Review
- The Hsp90 Chaperone Network Modulates Candida Virulence Traits.Trends in microbiology · 2017Review
- Regulatory roles of phosphorylation in model and pathogenic fungi.Medical mycology · 2016Review
- Cell cycle-independent phospho-regulation of Fkh2 during hyphal growth regulates Candida albicans pathogenesis.PLoS pathogens · 2015Article
- Molecular cloning, modeling and differential expression of a gene encoding a silent information regulator-like protein from Sporothrix schenckii.International journal of molecular medicine · 2014Article
- Roles of Candida albicans Gat2, a GATA-type zinc finger transcription factor, in biofilm formation, filamentous growth and virulence.PloS one · 2012Article
- Functional characterization of a new member of the Cdk9 family in Aspergillus nidulans.Eukaryotic cell · 2010Article
- Mss11, a transcriptional activator, is required for hyphal development in Candida albicans.Eukaryotic cell · 2009Article
- Roles of Candida albicans Sfl1 in hyphal development.Eukaryotic cell · 2007Article
- The Flo8 transcription factor is essential for hyphal development and virulence in Candida albicans.Molecular biology of the cell · 2006Article
- Regulation of the Cdc42/Cdc24 GTPase module during Candida albicans hyphal growth.Eukaryotic cell · 2005Article
- Susceptibility of germfree phagocyte oxidase- and nitric oxide synthase 2-deficient mice, defective in the production of reactive metabolites of both oxygen and nitrogen, to mucosal and systemic candidiasis of endogenous origin.Infection and immunity · 2005Article
- cDNA microarray analysis of differential gene expression in Candida albicans biofilm exposed to farnesol.Antimicrobial agents and chemotherapy · 2005Article
- Transcription profiling of cyclic AMP signaling in Candida albicans.Molecular biology of the cell · 2004Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Both mitogen-activated protein kinases and cyclin-dependent kinases play a role in hyphal development in Candida albicans. Using an oligonucleotide probe-based screen, we have isolated a new member of the Cdc2 kinase subfamily, designated Crk1 (Cdc2-related kinase). The protein sequence of Crk1 is most similar to those of Saccharomyces cerevisiae Sgv1 and human Pkl1/Cdk9. In S. cerevisiae, CRK1 suppresses some, but not all, of the defects associated with an sgv1 mutant. Deleting both copies of CRK1 in C. albicans slows growth slightly but leads to a profound defect in hyphal development under all conditions examined. crk1/crk1 mutants are impaired in the induction of hypha-specific genes and are avirulent in mice. Consistent with this, ectopic expression of the Crk1 kinase domain (CRK1N) promotes filamentous or invasive growth in S. cerevisiae and hyphal development in C. albicans. The activity of Crk1 in S. cerevisiae requires Flo8 but is independent of Ste12 and Phd1. Similarly, Crk1 promotes filamentation through a route independent of Cph1 and Efg1 in C. albicans. RAS1(V13) can also activate filamentation in a cph1/cph1 efg1/efg1 double mutant. Interestingly, CRK1N produces florid hyphae in ras1/ras1 strains, while RAS1(V13) generates feeble hyphae in crk1/crk1 strains.
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