ArticleJournal of bacteriology2000
Characterization of Borrelia burgdorferi BlyA and BlyB proteins: a prophage-encoded holin-like system.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Erp and Rev Adhesins of the Lyme Disease Spirochete's Ubiquitous cp32 Prophages Assist the Bacterium during Vertebrate Infection.Infection and immunity · 2023Review
- Structural Analysis of the Outer Membrane Lipoprotein BBA14 (OrfD) and the Corresponding Paralogous Gene Family 143 (PFam143) fromPathogens (Basel, Switzerland) · 2022Article
- Unconventional Approaches to Direct Detection of Borreliosis and Other Tick Borne Illnesses: A Path Forward.Journal of cellular immunology · 2021Article
- Article
- The Borrelia burgdorferi RelA/SpoT Homolog and Stringent Response Regulate Survival in the Tick Vector and Global Gene Expression during Starvation.PLoS pathogens · 2015Article
- Characterization of the RelBbu Regulon in Borrelia burgdorferi Reveals Modulation of Glycerol Metabolism by (p)ppGpp.PloS one · 2015Article
- Article
- Reliability of nine programs of topological predictions and their application to integral membrane channel and carrier proteins.Journal of molecular microbiology and biotechnology · 2014Article
- Peaceful coexistence amongst Borrelia plasmids: getting by with a little help from their friends?Plasmid · 2013Review
- An inverted repeat in the ospC operator is required for induction in Borrelia burgdorferi.PloS one · 2013Article
- Characterization of DLP12 Prophage Membrane Associated Protein: HolinGFP.Indian journal of microbiology · 2012Article
- B1b lymphocyte-derived antibodies control Borrelia hermsii independent of Fcα/μ receptor and in the absence of host cell contact.Immunologic research · 2011Review
- Lyme borreliosis spirochete Erp proteins, their known host ligands, and potential roles in mammalian infection.International journal of medical microbiology : IJMM · 2008Review
- Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette.Journal of biosciences · 2007Review
- Borrelia burgdorferi EbfC, a novel, chromosomally encoded protein, binds specific DNA sequences adjacent to erp loci on the spirochete's resident cp32 prophages.Journal of bacteriology · 2006Article
- Demonstration of cotranscription and 1-methyl-3-nitroso-nitroguanidine induction of a 30-gene operon of Borrelia burgdorferi: evidence that the 32-kilobase circular plasmids are prophages.Journal of bacteriology · 2005Article
- Identification of genes of VSH-1, a prophage-like gene transfer agent of Brachyspira hyodysenteriae.Journal of bacteriology · 2005Article
- Extracellular secretion of the Borrelia burgdorferi Oms28 porin and Bgp, a glycosaminoglycan binding protein.Infection and immunity · 2004Article
- Combined effects of blood and temperature shift on Borrelia burgdorferi gene expression as determined by whole genome DNA array.Infection and immunity · 2004Article
- Whole-genome DNA array analysis of the response of Borrelia burgdorferi to a bactericidal monoclonal antibody.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The conserved cp32 plasmid family of Borrelia burgdorferi was recently shown to be packaged into a bacteriophage particle (C. H. Eggers and D. S. Samuels, J. Bacteriol. 181:7308-7313, 1999). This plasmid encodes BlyA, a 7.4-kDa membrane-interactive protein, and BlyB, an accessory protein, which were previously proposed to comprise a hemolysis system. Our genetic and biochemical evidence suggests that this hypothesis is incorrect and that BlyA and BlyB function instead as a prophage-encoded holin or holin-like system for this newly described bacteriophage. An Escherichia coli mutant containing the blyAB locus that was defective for the normally cryptic host hemolysin SheA was found to be nonhemolytic, suggesting that induction of sheA by blyAB expression was responsible for the hemolytic activity observed previously. Analysis of the structural features of BlyA indicated greater structural similarity to bacteriophage-encoded holins than to hemolysins. Consistent with holin characteristics, subcellular localization studies with E. coli and B. burgdorferi indicated that BlyA is solely membrane associated and that BlyB is a soluble protein. Furthermore, BlyA exhibited a holin-like function by promoting the endolysin-dependent lysis of an induced lambda lysogen that was defective in the holin gene. Finally, induction of the cp32 prophage in B. burgdorferi dramatically stimulated blyAB expression. Our results provide the first evidence of a prophage-encoded holin within Borrelia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.