ArticleJournal of bacteriology2000
Catalase-peroxidases of Legionella pneumophila: cloning of the katA gene and studies of KatA function.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Analysis of the genes and mechanisms responsible for the cytotoxicity of theFrontiers in microbiology · 2025Article
- Effector-mediated subversion of proteasome activator (PA)28αβ enhances host defense against Legionella pneumophila under inflammatory and oxidative stress conditions.PLoS pathogens · 2023Article
- GVPC Medium Manufactured without Oxygen Improves the Growth ofMicrobiology spectrum · 2022Article
- Genomic analysis reveals high intra-species diversity ofMicrobial genomics · 2022Article
- Knowledge to Predict Pathogens:Microorganisms · 2022Review
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- Population structure and minimum core genome typing of Legionella pneumophila.Scientific reports · 2016Article
- Common Non-classically Secreted Bacterial Proteins with Experimental Evidence.Current microbiology · 2016Review
- The Legionella pneumophila kai operon is implicated in stress response and confers fitness in competitive environments.Environmental microbiology · 2014Article
- How are the non-classically secreted bacterial proteins released into the extracellular milieu?Current microbiology · 2013Review
- Implication of the VirD4 coupling protein of the Lvh type 4 secretion system in virulence phenotypes of Legionella pneumophila.Journal of bacteriology · 2013Article
- Molecular diversity and high virulence of Legionella pneumophila strains isolated from biofilms developed within a warm spring of a thermal spa.BMC microbiology · 2013Article
- Virulence phenotypes of Legionella pneumophila associated with noncoding RNA lpr0035.Infection and immunity · 2012Article
- Implication of proteins containing tetratricopeptide repeats in conditional virulence phenotypes of Legionella pneumophila.Journal of bacteriology · 2012Article
- Rhodococcus equi's extreme resistance to hydrogen peroxide is mainly conferred by one of its four catalase genes.PloS one · 2012Article
- Screening-level assays for potentially human-infectious environmental Legionella spp.Journal of microbiology (Seoul, Korea) · 2011Article
- An ortholog of OxyR in Legionella pneumophila is expressed postexponentially and negatively regulates the alkyl hydroperoxide reductase (ahpC2D) operon.Journal of bacteriology · 2008Article
- Environmental mimics and the Lvh type IVA secretion system contribute to virulence-related phenotypes of Legionella pneumophila.Infection and immunity · 2007Article
- Article
- Compensatory functions of two alkyl hydroperoxide reductases in the oxidative defense system of Legionella pneumophila.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila, the causative organism of Legionnaires' pneumonia, contains two enzymes with catalatic and peroxidatic activity, KatA and KatB. To address the issue of redundant, overlapping, or discrete in vivo functions of highly homologous catalase-peroxidases, the gene for katA was cloned and its function was studied in L. pneumophila and Escherichia coli and compared with prior studies of katB in this laboratory. katA is induced during exponential growth and is the predominant peroxidase in stationary phase. When katA is inactivated, L. pneumophila is more sensitive to exogenous hydrogen peroxide and less virulent in the THP-1 macrophage cell line, similar to katB. Catalatic-peroxidatic activity with different peroxidatic cosubstrates is comparable for KatA and KatB, but KatA is five times more active towards dianisidine. In contrast with these examples of redundant or overlapping function, stationary-phase survival is decreased by 100- to 10,000-fold when katA is inactivated, while no change from wild type is seen for the katB null. The principal clue for understanding this discrete in vivo function was the demonstration that KatA is periplasmic and KatB is cytosolic. This stationary-phase phenotype suggests that targets sensitive to hydrogen peroxide are present outside the cytosol in stationary phase or that the peroxidatic activity of KatA is critical for stationary-phase redox reactions in the periplasm, perhaps disulfide bond formation. Since starvation-induced stationary phase is a prerequisite to acquisition of virulence by L. pneumophila, further studies on the function and regulation of katA in stationary phase may give insights on the mechanisms of infectivity of this pathogen.
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