ArticleRNA (New York, N.Y.)2000
Detailed characterization of the posttranscriptional gene-silencing-related small RNA in a GUS gene-silenced tobacco.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Small RNAs in Plant Responses to Abiotic Stresses: Regulatory Roles and Study Methods.International journal of molecular sciences · 2015Review
- Delay in virus accumulation and low virus transmission from transgenic rice plants expressing Rice tungro spherical virus RNA.Virus genes · 2012Article
- Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene.Molecules and cells · 2012Article
- MicroRNAs in rhabdomyosarcoma: pathogenetic implications and translational potentiality.Molecular cancer · 2011Review
- A simple and efficient method for isolating small RNAs from different plant species.Plant methods · 2011Article
- Long double-stranded RNA produces specific gene downregulation in Giardia lamblia.The Journal of parasitology · 2010Article
- The infective cycle of Cabbage leaf curl virus (CaLCuV) is affected by CRUMPLED LEAF (CRL) gene in Arabidopsis thaliana.Virology journal · 2009Article
- RNA silencing against geminivirus: complementary action of posttranscriptional gene silencing and transcriptional gene silencing in host recovery.Journal of virology · 2009Article
- Article
- Generation of secondary small interfering RNA in cell-autonomous and non-cell autonomous RNA silencing in tobacco.Plant molecular biology · 2007Article
- The frequency and efficiency of endogene suppression by transitive silencing signals is influenced by the length of sequence homology.Plant physiology · 2006Article
- Transcriptionally silenced transgenes in maize are activated by three mutations defective in paramutation.Genetics · 2006Article
- The transcribed 165-bp CentO satellite is the major functional centromeric element in the wild rice species Oryza punctata.Plant physiology · 2005Article
- Evidence implying only unprimed RdRP activity during transitive gene silencing in plants.Plant molecular biology · 2005Article
- Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato.Plant cell reports · 2005Article
- Inhibition of hepatitis B virus surface antigen expression by small hairpin RNA in vitro.World journal of gastroenterology · 2005Article
- RNA silencing in plants by the expression of siRNA duplexes.Nucleic acids research · 2004Article
- A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.The Plant cell · 2004Article
- The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing.The Plant cell · 2003Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Posttranscriptional gene-silencing phenomena such as cosuppression and RNA interference are associated with the occurrence of small, about 21-23 nt short RNA species homologous to the silenced gene. We here show that the small RNA present in silenced transgenic plants can easily be detected in total RNA isolated according to standard procedures. This will allow for the development of routine and early screenings for the presence of small RNA species and, therefore, gene silencing in transgenic plants. We further demonstrate that the small RNA fraction can be visualized with the SYBR Green II RNA stain, isolated from a gel, labeled and used as a specific probe. Using these approaches, we have fine-mapped the sequences of the GUS gene that are represented in the small RNA fraction of a GUS-silenced tobacco line containing an inverted repeat of the GUS gene. In this tobacco line, the silencing-associated small RNA is a mixture of fragments that cover the 3' two-thirds of the GUS coding region. The 5' coding and the 3' noncoding ends of the GUS mRNA are not represented in the small RNA fraction. The RNA fragments are not likely to be a primary synthesis product of an RNA-dependent RNA polymerase, but rather degradation products from nuclease activity. Surprisingly, RNA isolated from wild-type, untransformed plants showed the presence of a similar-sized small RNA pool. This might indicate the existence of small RNA species from putative endogenous genes that are down regulated by a similar posttranscriptional gene-silencing mechanism. The possibility of isolating and labeling the small RNA pool from wild-type plants will provide a way to identify and study such putative genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.