ArticleNucleic acids research2000
Addition of non-genomically encoded nucleotides to the 3'-terminus of maize mitochondrial mRNAs: truncated rps12 mRNAs frequently terminate with CCA.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Rapid Shifts in Mitochondrial tRNA Import in a Plant Lineage with Extensive Mitochondrial tRNA Gene Loss.Molecular biology and evolution · 2021Article
- Hopeful monsters: unintended sequencing of famously malformed mite mitochondrial tRNAs reveals widespread expression and processing of sense-antisense pairs.NAR genomics and bioinformatics · 2021Article
- Exploration of CCA-added RNAs revealed the expression of mitochondrial non-coding RNAs regulated by CCA-adding enzyme.RNA biology · 2019Article
- Major contribution of transcription initiation to 5'-end formation of mitochondrial steady-state transcripts in maize.RNA biology · 2019Article
- The identity of the discriminator base has an impact on CCA addition.Nucleic acids research · 2015Article
- Mitochondrial atp9 genes from petaloid male-sterile and male-fertile carrots differ in their status of heteroplasmy, recombination involvement, post-transcriptional processing as well as accumulation of RNA and protein product.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.Cellular and molecular life sciences : CMLS · 2010Review
- Developmentally-specific transcripts from the ccmFN-rps1 locus in wheat mitochondria.Molecular genetics and genomics : MGG · 2008Article
- Mapping of mitochondrial mRNA termini in Arabidopsis thaliana: t-elements contribute to 5' and 3' end formation.Nucleic acids research · 2007Article
- Nontemplated nucleotide addition prior to polyadenylation: a comparison of Arabidopsis cDNA and genomic sequences.RNA (New York, N.Y.) · 2004Article
- Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.Nucleic acids research · 2004Article
- Synthesis and processing of tRNA-related SINE transcripts in Arabidopsis thaliana.Nucleic acids research · 2004Article
- Surprising features of plastid ndhD transcripts: addition of non-encoded nucleotides and polysome association of mRNAs with an unedited start codon.Nucleic acids research · 2004Article
- RT-PCR analysis of 5' to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria.Nucleic acids research · 2002Article
- Differential localization of nuclear-encoded tRNAs between the cytosol and mitochondrion in Leishmania tarentolae.RNA (New York, N.Y.) · 2002Article
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3 authors.
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Abstract
The 3'-termini of maize mitochondrial RNAs were characterized by ligation of an anchor oligonucleotide, reverse transcription and amplification. DNA sequence analysis of cDNA clones for tRNA(Ser) and 18S rRNA confirmed the expected 3'-terminal nucleotides and demonstrated the accuracy and fidelity of the protocol. Analysis of cDNAs for rps12, cox2 and atp9 indicated that non-genomically encoded nucleotides were present at the 3'-terminus. rps12 cDNAs exhibited the highest degree of modification, with 94% of 35 cDNA clones analyzed containing one to four non-genomically encoded C or A residues; 83% of these cDNAs terminated with the trinucleotide CCA. DNA sequence and transcript mapping analyses demonstrated that four positions exhibited modified 3'-termini within a small region of the 3' flank of rps12 transcripts. These transcript termini represented low abundance, truncated forms of rps12 mRNAs which may be intermediates in degradation. cox2 mRNAs are also modified at a truncated position. Sixty percent of the cox2 cDNAs were modified with 1-5 nt that most frequently included A and C residues, but also included a few G and T residues. Non-genomically encoded nucleotides were detected in 27% of the atp9 cDNAs as a single C or A residue.
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