ArticleMolecular biology of the cell2000
The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- TheCells · 2021Review
- Systematic Protein-Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex.Molecular & cellular proteomics : MCP · 2016Article
- A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.Biochimica et biophysica acta · 2013Article
- Structure, dynamics, evolution, and function of a major scaffold component in the nuclear pore complex.Structure (London, England : 1993) · 2013Article
- Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly.The EMBO journal · 2012Article
- The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.Cell & bioscience · 2012Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes.Molecular biology of the cell · 2012Article
- Hypoxia elicits broad and systematic changes in protein subcellular localization.American journal of physiology. Cell physiology · 2011Article
- Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex.Molecular biology of the cell · 2005Article
- Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating.Molecular and cellular biology · 2003Article
- In situ analysis of spatial relationships between proteins of the nuclear pore complex.Biophysical journal · 2002Article
- Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes.The Journal of cell biology · 2002Article
- A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of approximately 30 different proteins (nucleoporins). Pre-embedding immunogold-electron microscopy revealed that Nic96p, an essential yeast nucleoporin, is located about the cytoplasmic and the nuclear periphery of the central channel, and near or at the distal ring of the yeast NPC. Genetic approaches further implicated Nic96p in nuclear protein import. To more specifically explore the potential role of Nic96p in nuclear protein import, we performed a two-hybrid screen with NIC96 as the bait against a yeast genomic library to identify transport factors and/or nucleoporins involved in nuclear protein import interacting with Nic96p. By doing so, we identified the yeast nucleoporin Nup53p, which also exhibits multiple locations within the yeast NPC and colocalizes with Nic96p in all its locations. Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export.
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