ArticleMolecular biology of the cell2000
Epidermal growth factor receptor distribution during chemotactic responses.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 90 citations in OpenAlex.
- Emergent eukaryotic directional sensing via receptor degradation and diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021Review
- Integrating Mathematical Modeling with High-Throughput Imaging Explains How Polyploid Populations Behave in Nutrient-Sparse Environments.Cancer research · 2020Article
- Chemotaxis Model for Breast Cancer Cells Based on Signal/Noise Ratio.Biophysical journal · 2018Article
- Targeting de novo lipogenesis as a novel approach in anti-cancer therapy.British journal of cancer · 2018Article
- Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch.Journal of the American Heart Association · 2016Article
- A Phosphatidic Acid (PA) conveyor system of continuous intracellular transport from cell membrane to nucleus maintains EGF receptor homeostasis.Oncotarget · 2016Article
- Towards comprehensive cardiac repair and regeneration after myocardial infarction: Aspects to consider and proteins to deliver.Biomaterials · 2016Review
- PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena.Molecular biology of the cell · 2015Article
- Tandem phosphorylation within an intrinsically disordered region regulates ACTN4 function.Science signaling · 2015Article
- Article
- An on-chip study on the influence of geometrical confinement and chemical gradient on cell polarity.Biomicrofluidics · 2014Article
- Polarised clathrin-mediated endocytosis of EGFR during chemotactic invasion.Traffic (Copenhagen, Denmark) · 2014Article
- Quantitative analysis of gradient sensing: towards building predictive models of chemotaxis in cancer.Current opinion in cell biology · 2012Review
- Epithelial cell guidance by self-generated EGF gradients.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.Physiological reviews · 2012Review
- Developing a multiscale, multi-resolution agent-based brain tumor model by graphics processing units.Theoretical biology & medical modelling · 2011Article
- Tyrosine phosphorylation of the Gα-interacting protein GIV promotes activation of phosphoinositide 3-kinase during cell migration.Science signaling · 2011Article
- Chemotaxis in cancer.Nature reviews. Cancer · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
To determine the distribution of the epidermal growth factor (EGF) receptor (EGFR) on the surface of cells responding to EGF as a chemoattractant, an EGFR-green fluorescent protein chimera was expressed in the MTLn3 mammary carcinoma cell line. The chimera was functional and easily visualized on the cell surface. In contrast to other studies indicating that the EGFR might be localized to certain regions of the plasma membrane, we found that the chimera is homogeneously distributed on the plasma membrane and becomes most concentrated in vesicles after endocytosis. In spatial gradients of EGF, endocytosed receptor accumulates on the upgradient side of the cell. Visualization of the binding of fluorescent EGF to cells reveals that the affinity properties of the receptor, together with its expression level on cells, can provide an initial amplification step in spatial gradient sensing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.