ArticleMolecular biology of the cell2000
Factors affecting nuclear export of the 60S ribosomal subunit in vivo.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 138 citations in OpenAlex.
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly.Nature communications · 2023Article
- The RGG motif proteins: Interactions, functions, and regulations.Wiley interdisciplinary reviews. RNA · 2023Review
- Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.Nucleic acids research · 2022Article
- Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Tracing Eukaryotic Ribosome Biogenesis Factors Into the Archaeal Domain Sheds Light on the Evolution of Functional Complexity.Frontiers in microbiology · 2021Article
- Article
- The chemical diversity of RNA modifications.The Biochemical journal · 2019Review
- RNA modifications and the link to human disease.Methods in enzymology · 2019Review
- Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 inThe Journal of biological chemistry · 2017Article
- The T-cell leukemia related rpl10-R98S mutant traps the 60S export adapter Nmd3 in the ribosomal P site in yeast.PLoS genetics · 2017Article
- Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1.Nature structural & molecular biology · 2017Article
- Principles of 60S ribosomal subunit assembly emerging from recent studies in yeast.The Biochemical journal · 2017Review
- Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.PloS one · 2016Article
- Granules harboring translationally active mRNAs provide a platform for P-body formation following stress.Cell reports · 2014Article
- Review
- Characterization of karyopherins in androgen receptor intracellular trafficking in the yeast model.International journal of clinical and experimental pathology · 2014Article
- Ribosome biogenesis in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- Regulation of ribosome biogenesis by nucleostemin 3 promotes local and systemic growth in Drosophila.Genetics · 2013Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Saccharomyces cerevisiae, the 60S ribosomal subunit assembles in the nucleolus and then is exported to the cytoplasm, where it joins the 40S subunit for translation. Export of the 60S subunit from the nucleus is known to be an energy-dependent and factor-mediated process, but very little is known about the specifics of its transport. To begin to address this problem, an assay was developed to follow the localization of the 60S ribosomal subunit in S. cerevisiae. Ribosomal protein L11b (Rpl11b), one of the approximately 45 ribosomal proteins of the 60S subunit, was tagged at its carboxyl terminus with the green fluorescent protein (GFP) to enable visualization of the 60S subunit in living cells. A panel of mutant yeast strains was screened for their accumulation of Rpl11b-GFP in the nucleus as an indicator of their involvement in ribosome synthesis and/or transport. This panel included conditional alleles of several rRNA-processing factors, nucleoporins, general transport factors, and karyopherins. As predicted, conditional alleles of rRNA-processing factors that affect 60S ribosomal subunit assembly accumulated Rpl11b-GFP in the nucleus. In addition, several of the nucleoporin mutants as well as a few of the karyopherin and transport factor mutants also mislocalized Rpl11b-GFP. In particular, deletion of the previously uncharacterized karyopherin KAP120 caused accumulation of Rpl11b-GFP in the nucleus, whereas ribosomal protein import was not impaired. Together, these data further define the requirements for ribosomal subunit export and suggest a biological function for KAP120.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.