ArticleProceedings of the National Academy of Sciences of the United States of America2000
Identification of alternative splicing and negative splicing activity of a nonsegmented negative-strand RNA virus, Borna disease virus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- ContinuousVirus evolution · 2026Article
- Wǔhàn sharpbelly bornavirus infects and persists in cypriniform cells.Journal of virology · 2025Article
- Reverse genetics of parrot bornavirus 4 reveals a unique splicing of the glycoprotein gene that affects viral propagation.Journal of virology · 2023Article
- Identification of RNA Virus-Derived RdRp Sequences in Publicly Available Transcriptomic Data Sets.Molecular biology and evolution · 2023Article
- Diving deep into fish bornaviruses: Uncovering hidden diversity and transcriptional strategies through comprehensive data mining.Virus evolution · 2023Article
- Article
- Circular RNAs: New Epigenetic Signatures in Viral Infections.Frontiers in microbiology · 2020Review
- Splicing-Dependent Subcellular Targeting of Borna Disease Virus Nucleoprotein Isoforms.Journal of virology · 2019Article
- A novel borna disease virus vector system that stably expresses foreign proteins from an intercistronic noncoding region.Journal of virology · 2011Article
- RNA splicing in a new rhabdovirus from Culex mosquitoes.Journal of virology · 2011Article
- Recovery of divergent avian bornaviruses from cases of proventricular dilatation disease: identification of a candidate etiologic agent.Virology journal · 2008Article
- Reverse-genetic approaches to the study of Borna disease virus.Nature reviews. Microbiology · 2006Review
- MAASE: an alternative splicing database designed for supporting splicing microarray applications.RNA (New York, N.Y.) · 2005Article
- Identification of splicing silencers and enhancers in sense Alus: a role for pseudoacceptors in splice site repression.Molecular and cellular biology · 2005Article
- Functional characterization of the genomic promoter of borna disease virus (BDV): implications of 3'-terminal sequence heterogeneity for BDV persistence.Journal of virology · 2005Article
- The use of peptide arrays for the characterization of monospecific antibody repertoires from polyclonal sera of psychiatric patients suspected of infection by Borna Disease Virus.Molecular diversity · 2004Article
- Borna disease virus.Journal of neurovirology · 2003Review
- RNA splicing at human immunodeficiency virus type 1 3' splice site A2 is regulated by binding of hnRNP A/B proteins to an exonic splicing silencer element.Journal of virology · 2001Article
- Regulation of alternative RNA splicing by exon definition and exon sequences in viral and mammalian gene expression.Journal of biomedical scienceReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Borna disease virus (BDV) is a nonsegmented negative-strand RNA virus that belongs to the Mononegavirales. Unlike other animal viruses of this order, BDV replicates and transcribes in the nucleus of infected cells. Previous studies have shown that BDV uses RNA splicing machinery for its mRNA expression. In the present study, we identified spliced RNAs that use an alternative 3' splice site, SA3, in BDV-infected cell lines as well as infected animal brain cells. Transient transfection analysis of cDNA clones of BDV RNA revealed that although SA3 is a favorable splice site in mammalian cells, utilization of SA3 is negatively regulated in infected cells. This negative splicing activity of the SA3 site is regulated by a putative cis-acting region, the exon splicing suppressor (ESS), within the polymerase exon of BDV. The BDV ESS contains similar motifs to other known ESSs present in viral and cellular genes. Furthermore, our results indicated that a functional polyadenylation signal just upstream of the BDV ESS is also involved in the regulation of alternative splicing of BDV. These observations represent the first documentation of complex RNA splicing in animal RNA viruses and also provide new insight into the mechanism of regulation of alternative splicing in animal viruses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.