ArticleProceedings of the National Academy of Sciences of the United States of America2000
Interaction of yeast kinetochore proteins with centromere-protein/transcription factor Cbf1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 39 citations in OpenAlex.
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.bioRxiv : the preprint server for biology · 2026Article
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- A transcriptional roadblock protects yeast centromeres.Nucleic acids research · 2022Article
- FBXO38 Ubiquitin Ligase Controls Centromere Integrity via ZXDA/B Stability.Frontiers in cell and developmental biology · 2022Article
- Article
- Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.Current biology : CB · 2019Article
- Multiple transcription factors contribute to inter-chromosomal interaction in yeast.BMC systems biology · 2018Article
- Centromere Biology: Transcription Goes on Stage.Molecular and cellular biology · 2018Review
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Reconstitution of hemisomes on budding yeast centromeric DNA.Nucleic acids research · 2013Article
- Article
- Transcription in the maintenance of centromere chromatin identity.Nucleic acids research · 2012Review
- Tripartite organization of centromeric chromatin in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Endogenous transcription at the centromere facilitates centromere activity in budding yeast.Current biology : CB · 2011Article
- Article
- Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast.Molecular cell · 2011Article
- The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres.The Journal of biological chemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centromere-kinetochore complex of Saccharomyces cerevisiae is a specialized chromosomal substructure that mediates attachment of duplicated chromosomes to the mitotic spindle by a regulated network of protein-DNA and protein-protein interactions. We have used in vitro assays to analyze putative molecular interactions between components of the yeast centromerekinetochore complex. Glutathione S-transferase pull-down experiments showed the direct interaction of in vitro translated p110, p64, and p58 of the essential CBF3 kinetochore protein complex with Cbf1p, a basic region helix-loop-helix zipper protein (bHLHzip) that specifically binds to the CDEI region on the centromere DNA. Furthermore, recombinant p64 and p23 each stimulated the in vitro DNA binding activity of Cbf1p. The N-terminal 70 amino acids of p23 were sufficient to mediate this effect. P64 could also promote the multimerization activity of Cbf1p in the presence of centromere DNA in vitro. These results show the direct physical interaction of Cbf1p and CBF3 subunits and provide evidence that CBF3 components can promote the binding of Cbf1p to its binding site in the yeast kinetochore. A functional comparison of the centromere binding proteins with transcription factors binding at MET16 promoters reveals the strong analogy between centromeres and the MET16 promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.