ArticleProceedings of the National Academy of Sciences of the United States of America2000
Polymerization of nontemplate bases before transcription initiation at the 3' ends of templates by an RNA-dependent RNA polymerase: an activity involved in 3' end repair of viral RNAs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Emergency Services of Viral RNAs: Repair and Remodeling.Microbiology and molecular biology reviews : MMBR · 2018Review
- Structural Components for Amplification of Positive and Negative Strand VEEV Splitzicons.Frontiers in molecular biosciences · 2018Article
- The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.Journal of virology · 2015Article
- Common and unique features of viral RNA-dependent polymerases.Cellular and molecular life sciences : CMLS · 2014Review
- Flock house virus RNA polymerase initiates RNA synthesis de novo and possesses a terminal nucleotidyl transferase activity.PloS one · 2014Article
- Characterization of a nodavirus replicase revealed a de novo initiation mechanism of RNA synthesis and terminal nucleotidyltransferase activity.The Journal of biological chemistry · 2013Article
- Molecular basis for nucleotide conservation at the ends of the dengue virus genome.PLoS pathogens · 2012Article
- Helper virus-independent transcription and multimerization of a satellite RNA associated with cucumber mosaic virus.Journal of virology · 2012Article
- Role of RNase MRP in viral RNA degradation and RNA recombination.Journal of virology · 2011Article
- The combined effect of environmental and host factors on the emergence of viral RNA recombinants.PLoS pathogens · 2010Article
- Bunyamwera virus can repair both insertions and deletions during RNA replication.RNA (New York, N.Y.) · 2010Article
- Genome 3'-end repair in dengue virus type 2.RNA (New York, N.Y.) · 2008Article
- Catalytic core of alphavirus nonstructural protein nsP4 possesses terminal adenylyltransferase activity.Journal of virology · 2006Article
- Different de novo initiation strategies are used by influenza virus RNA polymerase on its cRNA and viral RNA promoters during viral RNA replication.Journal of virology · 2006Article
- Conformational changes involved in initiation of minus-strand synthesis of a virus-associated RNA.RNA (New York, N.Y.) · 2006Article
- The AAUAAA motif of bamboo mosaic virus RNA is involved in minus-strand RNA synthesis and plus-strand RNA polyadenylation.Journal of virology · 2005Article
- Genome-wide screen identifies host genes affecting viral RNA recombination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Repair of the tRNA-like CCA sequence in a multipartite positive-strand RNA virus.Journal of virology · 2005Article
- Diverse Mechanisms of RNA Recombination.Molecular biology · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 3' ends of RNAs associated with turnip crinkle virus (TCV), including subviral satellite (sat)C, terminate with the motif CCUGCCC-3'. Transcripts of satC with a deletion of the motif are repaired to wild type (wt) in vivo by RNA-dependent RNA polymerase (RdRp)-mediated extension of abortively synthesized oligoribonucleotide primers complementary to the 3' end of the TCV genomic RNA. Repair of shorter deletions, however, are repaired by other mechanisms. SatC transcripts with the 3' terminal CCC replaced by eight nonviral bases were repaired in plants by homologous recombination between the similar 3' ends of satC and TCV. Transcripts with deletions of four or five 3' terminal bases, in the presence or absence of nonviral bases, generated progeny with a mixture of wt and non-wt 3' ends in vivo. In vitro, RdRp-containing extracts were able to polymerize nucleotides in a template-independent fashion before using these primers to initiate transcription at or near the 3' end of truncated satC templates. The nontemplate additions at the 5' ends of the nascent complementary strands were not random, with a preference for consecutive identical nucleotides. The RdRp was also able to initiate transcription opposite cytidylate, uridylate, guanylate, and possibly adenylate residues without exhibiting an obvious preference, flexibility previously unreported for viral RdRp. The unexpected existence of three different repair mechanisms for TCV suggests that 3' end reconstruction is critical to virus survival.
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