ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Intrinsic membrane properties underlying spontaneous tonic firing in neostriatal cholinergic interneurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 182 papers.
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Who cites it
182 citing papers in PubMed.
- HCAR1-mediated lactate signaling modulates motor behavior and regulates spontaneous firing in Purkinje cells.iScience · 2026Article
- Morphoelectric Diversity and Specialization of Neuronal Cell Types in the Primate Striatum.bioRxiv : the preprint server for biology · 2026Article
- Altered striosome-matrix distribution and activity of striatal cholinergic interneurons in a model of autism-linked repetitive behaviors.Molecular psychiatry · 2026Article
- Regional heterogeneity of striatal cholinergic interneurons: setting the stage for diverse behavioral repertoires.Frontiers in cellular neuroscience · 2026Review
- Levodopa Disrupts Activity Patterns and Encoding of Movement in Striatal Cholinergic Interneurons of Behaving Mice.Movement disorders : official journal of the Movement Disorder Society · 2025Article
- An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.bioRxiv : the preprint server for biology · 2025Article
- Pregnenolone sulfate potentiates tetrodotoxin-resistant NaThe journal of headache and pain · 2025Article
- Extrinsic and intrinsic control of striatal cholinergic interneuron activity.Frontiers in molecular neuroscience · 2025Review
- A detailed look at striatal acetylcholine, dopamine, and their interactions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Noise or signal? Spontaneous activity of dorsal horn neurons: patterns and function in health and disease.Pflugers Archiv : European journal of physiology · 2024Review
- Corticotropin releasing factor alters the functional diversity of accumbal cholinergic interneurons.Journal of neurophysiology · 2024Article
- Glycine-induced activation of GPR158 increases the intrinsic excitability of medium spiny neurons in the nucleus accumbens.Cellular and molecular life sciences : CMLS · 2024Article
- Catharanthine Modulates Mesolimbic Dopamine Transmission and Nicotine Psychomotor Effects via Inhibition of α6-Nicotinic Receptors and Dopamine Transporters.ACS chemical neuroscience · 2024Article
- Comparing tonic and phasic dendritic calcium in cholinergic pedunculopontine neurons and dopaminergic substantia nigra neurons.The European journal of neuroscience · 2024Article
- A sex-specific effect of MFrontiers in molecular neuroscience · 2024Article
- Regional heterogeneity in the membrane properties of mouse striatal neurons.Frontiers in cellular neuroscience · 2024Article
- Article
- The early excitatory action of striatal cholinergic-GABAergic microcircuits conditions the subsequent GABA inhibitory shift.Communications biology · 2023Article
- Cognitive effects of thalamostriatal degeneration are ameliorated by normalizing striatal cholinergic activity.Science advances · 2023Article
- Role of α6-Nicotinic Receptors in Alcohol-Induced GABAergic Synaptic Transmission and Plasticity to Cholinergic Interneurons in the Nucleus Accumbens.Molecular neurobiology · 2023Article
122 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Neostriatal cholinergic interneurons produce spontaneous tonic firing in the absence of synaptic input. Perforated patch recording and whole-cell recording combined with calcium imaging were used in vitro to identify the intrinsic membrane properties underlying endogenous excitability. Spontaneous firing was driven by the combined action of a sodium current and the hyperpolarization-activated cation current (I(h)), which together ensured that there was no zero current point in the subthreshold voltage range. Blockade of sodium channels or I(h) established a stable subthreshold resting membrane potential. A tetrodotoxin-sensitive region of negative slope conductance was observed between approximately -60 mV and threshold (approximately -50 mV) and the h-current was activated at all subthreshold voltages. Calcium imaging experiments revealed that there was minimal calcium influx at subthreshold membrane potentials but that action potentials produced elevations of calcium in both the soma and dendrites. Spike-triggered calcium entry shaped the falling phase of the action potential waveform and activated calcium-dependent potassium channels. Blockade of big-conductance channels caused spike broadening. Application of apamin, which blocks small-conductance channels, abolished the slow spike afterhyperpolarization (AHP) and caused a transition to burst firing. In the absence of synaptic input, a range of tonic firing patterns are observed, suggesting that the characteristic spike sequences described for tonically active cholinergic neurons (TANs) recorded in vivo are intrinsic in origin. The pivotal role of the AHP in regulating spike patterning indicates that burst firing of TANs in vivo could arise from direct or indirect modulation of the AHP without requiring phasic synaptic input.
Indexed as
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11069957PMC6773196What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.