ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Two independent pathways mediated by cAMP and protein kinase A enhance spontaneous transmitter release at Drosophila neuromuscular junctions.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Adrenergic Modulation of Acetylcholine Release at the Mouse Neuromuscular Junctions of Fast-Twitch Skeletal Muscle.Neurochemical research · 2025Review
- Action potential-independent spontaneous microdomain CaProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Repair of traumatic lesions to the plasmalemma of neurons and other cells: Commonalities, conflicts, and controversies.Frontiers in physiology · 2023Review
- Alteration in information flow through a pair of feeding command neurons underlies a form of Pavlovian conditioning in the Drosophila brain.Current biology : CB · 2021Article
- Synaptotagmin 7 switches short-term synaptic plasticity from depression to facilitation by suppressing synaptic transmission.Scientific reports · 2021Article
- Calcimimetic acts on enteric neuronal CaSR to reverse cholera toxin-induced intestinal electrolyte secretion.Scientific reports · 2018Article
- Molecular Mechanisms of Short-Term Plasticity: Role of Synapsin Phosphorylation in Augmentation and Potentiation of Spontaneous Glutamate Release.Frontiers in synaptic neuroscience · 2018Article
- Cyclic nucleotide signaling is required during synaptic refinement at the Drosophila neuromuscular junction.Developmental neurobiology · 2017Article
- Repair of traumatic plasmalemmal damage to neurons and other eukaryotic cells.Neural regeneration research · 2016Review
- Role of protein kinase A in regulating mitochondrial function and neuronal development: implications to neurodegenerative diseases.Reviews in the neurosciences · 2015Review
- Simultaneous recording of calcium signals from identified neurons and feeding behavior of Drosophila melanogaster.Journal of visualized experiments : JoVE · 2012Article
- A model for sealing plasmalemmal damage in neurons and other eukaryotic cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Differential regulation of synchronous versus asynchronous neurotransmitter release by the C2 domains of synaptotagmin 1.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Equilibrative nucleoside transporter 2 regulates associative learning and synaptic function in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- PKCgamma-induced trafficking of AMPA receptors in embryonic zebrafish depends on NSF and PICK1.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Increased Ca2+ influx through Na+/Ca2+ exchanger during long-term facilitation at crayfish neuromuscular junctions.The Journal of physiology · 2007Article
- D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates spontaneous neurotransmitter release.The Journal of cell biology · 2007Article
- Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cAMP is thought to be involved in learning process and known to enhance transmitter release in various systems. Previously we reported that cAMP enhances spontaneous transmitter release in the absence of extracellular Ca(2+) and that the synaptic vesicle protein neuronal-synaptobrevin (n-syb), is required in this enhancement (n-syb-dependent; Yoshihara et al., 1999). In the present study, we examined the cAMP-induced enhancement of transmitter release in the presence of external Ca(2+). We raised the intracellular concentration of cAMP by application of either forskolin, an activator of adenylyl cyclase, or by 4-chlorophenylthio-(CPT)-cAMP, a membrane-permeable analog of cAMP, in the presence of external Ca(2+), while recording miniature synaptic currents (mSCs) at the neuromuscular junction in n-syb null mutant embryos. The frequency of mSCs increased in response to elevation of cAMP, and this effect of cAMP was completely blocked by Co(2+) (n-syb-independent pathway). In contrast, in wild-type embryos the cAMP-induced mSC frequency increase was partially blocked by Co(2+). In a mutant, DC0, defective in protein kinase A (PKA), nerve-evoked synaptic currents were indistinguishable from the control, but mSCs were less frequent. In this mutant the enhancement by cAMP of both nerve-evoked and spontaneous transmitter release was completely absent, even in the presence of external Ca(2+). Taken together, these results suggest that cAMP enhances spontaneous transmitter release by increasing Ca(2+) influx (n-syb-independent) as well as by modulating the release mechanism without Ca(2+) influx (n-syb-dependent) in wild-type embryos, and these two effects are mediated by PKA encoded by the DC0 gene.
Indexed as
Identifiers
11069938PMC6773197W1594082692What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.