ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
The products of the Drosophila stoned locus interact with synaptic vesicles via synaptotagmin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- A novel dual Ca2+ sensor system regulates Ca2+-dependent neurotransmitter release.The Journal of cell biology · 2021Article
- Endocytic Adaptor Proteins in Health and Disease: Lessons from Model Organisms and Human Mutations.Cells · 2019Review
- SNT-1 Functions as the CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Safeguards of Neurotransmission: Endocytic Adaptors as Regulators of Synaptic Vesicle Composition and Function.Frontiers in cellular neuroscience · 2017Review
- Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.G3 (Bethesda, Md.) · 2015Article
- AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles.Traffic (Copenhagen, Denmark) · 2014Article
- Epsin 1 Promotes Synaptic Growth by Enhancing BMP Signal Levels in Motoneuron Nuclei.PloS one · 2013Article
- UNC-41/stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans.PloS one · 2012Article
- Ca2+ regulates the Drosophila Stoned-A and Stoned-B proteins interaction with the C2B domain of Synaptotagmin-1.PloS one · 2012Article
- AP180 and CALM: Dedicated endocytic adaptors for the retrieval of synaptobrevin 2 at synapses.Cellular logistics · 2011Article
- Tweek, an evolutionarily conserved protein, is required for synaptic vesicle recycling.Neuron · 2009Article
- Stoned B mediates sorting of integral synaptic vesicle proteins.Neuroscience · 2008Article
- Genetic modifiers of Drosophila palmitoyl-protein thioesterase 1-induced degeneration.Genetics · 2007Article
- Molecular and genetic characterization of the interactions between the Drosophila stoned-B protein and DAP-160 (intersectin).The Biochemical journal · 2005Article
- Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Article
- Role of synaptotagmin in Ca2+-triggered exocytosis.The Biochemical journal · 2002Review
- Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I.The Biochemical journal · 2002Article
- Adaptins: the final recount.Molecular biology of the cell · 2001Article
- Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating.EMBO reports · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The stoned locus of Drosophila melanogaster encodes two novel proteins, stonedA (STNA) and stonedB (STNB), both of which are expressed in the nervous system. Flies with defects at the stoned locus have abnormal behavior and altered synaptic transmission. Genetic interactions, in particular with the shibire (dynamin) mutation, indicated a presynaptic function for stoned and suggested an involvement in vesicle cycling. Immunological studies revealed colocalization of the stoned proteins at the neuromuscular junction with the integral synaptic vesicle protein synaptotagmin (SYT). We show here that stoned interacts genetically with synaptotagmin to produce a lethal phenotype. The STNB protein is found by co-immunoprecipitation to be associated with synaptic vesicles, and glutathione S-transferase pull-downs demonstrate an in vitro interaction between the micro2-homology domain of STNB and the C2B domain of the SYTI isoform. The STNA protein is also found in association with vesicles, and it too exhibits an in vitro association with SYTI. However, we find that the bulk of STNA is in a nonmembranous fraction. By using the shibire mutant to block endocytosis, STNB is shown to be present on some synaptic vesicles before exocytosis. However, STNB is not associated with all synaptic vesicles. We hypothesize that STNB specifies a subset of synaptic vesicles with a role in the synaptic vesicle cycle that is yet to be determined.
Indexed as
Identifiers
11069931PMC6773190W1569548564What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.