ArticleGenes & development2000
Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 148 papers.
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148 citing papers in PubMed, 278 citations in OpenAlex.
- Chromosome segregation synchrony in S. pombe is noise limited and arises without positive feedback.The Journal of cell biology · 2026Article
- Establishing MS2-MCP-based single-molecule RNA visualization in Schizosaccharomyces pombe.Journal of cell science · 2026Article
- Effects ofExperimental and therapeutic medicine · 2026Article
- Chromosome segregation synchrony inbioRxiv : the preprint server for biology · 2026Article
- A role for condensin-mediator interaction in mitotic chromosome organization.Nature communications · 2026Article
- Site-to-site mutational dissection of fission yeast cohesin reveals its dynamics.G3 (Bethesda, Md.) · 2025Article
- Unraveling the cohesin-chromatin interface: identifying protein interactions that modulate chromosome structure and function.Epigenetics & chromatin · 2025Article
- Sororin is an evolutionary conserved antagonist of WAPL.Nature communications · 2024Article
- Acetylation of Rec8 cohesin complexes regulates reductional chromosome segregation in meiosis.Life science alliance · 2024Article
- Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I.Life science alliance · 2024Article
- Condensin positioning at telomeres by shelterin proteins drives sister-telomere disjunction in anaphase.eLife · 2023Article
- Cohesin contributes to transcriptional repression of stage-specific genes in the human malaria parasite.EMBO reports · 2023Article
- Global Gene Expression of Post-Senescent Telomerase-NegativeJournal of fungi (Basel, Switzerland) · 2023Article
- BiFCo: visualizing cohesin assembly/disassembly cycle in living cells.Life science alliance · 2023Article
- Cohesin organization, dynamics, and subdomain functions revealed by genetic suppressor screening.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2023Article
- The Green Valley ofCells · 2022Review
- Single amino acid substitutions in hydrophobic cores at a head-coiled coil junction region of cohesin facilitate its release of DNA during anaphase.Open biology · 2022Article
- Force by minus-end motors Dhc1 and Klp2 collapses the S. pombe spindle after laser ablation.Biophysical journal · 2022Article
- Perturbation of kinetochore function using GFP-binding protein in fission yeast.G3 (Bethesda, Md.) · 2021Article
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88 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cohesin complex acts in the formation and maintenance of sister chromatid cohesion during and after S phase. Budding yeast Scc1p/Mcd1p, an essential subunit, is cleaved and dissociates from chromosomes in anaphase, leading to sister chromatid separation. Most cohesin in higher eukaryotes, in contrast, is dissociated from chromosomes well before anaphase. The universal role of cohesin during anaphase thus remains to be determined. We report here initial characterization of four putative cohesin subunits, Psm1, Psm3, Rad21, and Psc3, in fission yeast. They are essential for sister chromatid cohesion. Immunoprecipitation demonstrates stable complex formation of Rad21 with Psm1 and Psm3 but not with Psc3. Chromatin immunoprecipitation shows that cohesin subunits are enriched in broad centromere regions and that the level of centromere-associated Rad21 did not change from metaphase to anaphase, very different from budding yeast. In contrast, Rad21 containing similar cleavage sites to those of Scc1p/Mcd1p is cleaved specifically in anaphase. This cleavage is essential, although the amount of cleaved product is very small (<5%). Mis4, another sister chromatid cohesion protein, plays an essential role for loading Rad21 on chromatin. A simple model is presented to explain the specific behavior of fission yeast cohesin and why only a tiny fraction of Rad21 is sufficient to be cleaved for normal anaphase.
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