ArticleProceedings of the National Academy of Sciences of the United States of America2000
Apical sorting of a voltage- and Ca2+-activated K+ channel alpha -subunit in Madin-Darby canine kidney cells is independent of N-glycosylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 107 citations in OpenAlex.
- Lipid trapping slows ball-and-chain inactivation in a calcium-activated potassium channel.Nature communications · 2026Article
- Cholesterol, PtdIns(4,5)PbioRxiv : the preprint server for biology · 2026Article
- Anti-P antibodies that impair memory perturb hippocampal glutamatergic receptor trafficking, synapse structure and microglia.Molecular medicine (Cambridge, Mass.) · 2025Article
- The Slo1 Y450F Substitution Modifies Basal Function and Cholesterol Response of Middle Cerebral Artery Smooth Muscle BK Channels in a Sexually Dimorphic Manner.International journal of molecular sciences · 2025Article
- Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Frontiers in cell and developmental biology · 2022Article
- The influence of membrane bilayer thickness on KcsA channel activity.Channels (Austin, Tex.) · 2019Article
- Article
- The unique N-terminal sequence of the BKCa channel α-subunit determines its modulation by β-subunits.PloS one · 2017Article
- Functional insights into modulation of BKCa channel activity to alter myometrial contractility.Frontiers in physiology · 2014Review
- Regulation of potassium channel trafficking in the distal nephron.Current opinion in nephrology and hypertension · 2013Review
- Stiffened lipid platforms at molecular force foci.Proceedings of the National Academy of Sciences of the United States of America · 2013Review
- Large conductance, calcium- and voltage-gated potassium (BK) channels: regulation by cholesterol.Pharmacology & therapeutics · 2012Review
- N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.Molecular biology of the cell · 2011Article
- Smooth muscle cholesterol enables BK β1 subunit-mediated channel inhibition and subsequent vasoconstriction evoked by alcohol.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat.Pflugers Archiv : European journal of physiology · 2011Article
- Cholesterol depletion alters amplitude and pharmacology of vascular calcium-activated chloride channels.Cardiovascular research · 2010Article
- Cholesterol and ion channels.Sub-cellular biochemistry · 2010Review
- Clathrin and AP1B: key roles in basolateral trafficking through trans-endosomal routes.FEBS letters · 2009Review
- The beta1 subunit of Na+/K+-ATPase interacts with BKCa channels and affects their steady-state expression on the cell surface.FEBS letters · 2009Article
- The role of cell cholesterol and the cytoskeleton in the interaction between IK1 and maxi-K channels.American journal of physiology. Cell physiology · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The voltage- and Ca(2+)-activated K(+) (K(V,Ca)) channel is expressed in a variety of polarized epithelial cells seemingly displaying a tissue-dependent apical-to-basolateral regionalization, as revealed by electrophysiology. Using domain-specific biotinylation and immunofluorescence we show that the human channel K(V,Ca) alpha-subunit (human Slowpoke channel, hSlo) is predominantly found in the apical plasma membrane domain of permanently transfected Madin-Darby canine kidney cells. Both the wild-type and a mutant hSlo protein lacking its only potential N-glycosylation site were efficiently transported to the cell surface and concentrated in the apical domain even when they were overexpressed to levels 200- to 300-fold higher than the density of intrinsic Slo channels. Furthermore, tunicamycin treatment did not prevent apical segregation of hSlo, indicating that endogenous glycosylated proteins (e.g., K(V,Ca) beta-subunits) were not required. hSlo seems to display properties for lipid-raft targeting, as judged by its buoyant distribution in sucrose gradients after extraction with either detergent or sodium carbonate. The evidence indicates that the hSlo protein possesses intrinsic information for transport to the apical cell surface through a mechanism that may involve association with lipid rafts and that is independent of glycosylation of the channel itself or an associated protein. Thus, this particular polytopic model protein shows that glycosylation-independent apical pathways exist for endogenous membrane proteins in Madin-Darby canine kidney cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.