ArticleProceedings of the National Academy of Sciences of the United States of America2000
A CBF5 mutation that disrupts nucleolar localization of early tRNA biosynthesis in yeast also suppresses tRNA gene-mediated transcriptional silencing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- RNA polymerase II (RNAP II)-associated factors are recruited to tRNA loci, revealing that RNAP II- and RNAP III-mediated transcriptions overlap in yeast.The Journal of biological chemistry · 2019Article
- New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis.Mycopathologia · 2019Article
- Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression.Nature communications · 2019Article
- Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons.Nucleic acids research · 2018Article
- Identification of factors that promote biogenesis of tRNARNA biology · 2018Article
- Aggregation of Mod5 is affected by tRNA binding with implications for tRNA gene-mediated silencing.FEBS letters · 2017Article
- A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere.PLoS genetics · 2016Article
- DNA loops generate intracentromere tension in mitosis.The Journal of cell biology · 2015Article
- Argonaute 2 Binds Directly to tRNA Genes and Promotes Gene Repression in cis.Molecular and cellular biology · 2015Article
- Review
- Compromised RNA polymerase III complex assembly leads to local alterations of intergenic RNA polymerase II transcription in Saccharomyces cerevisiae.BMC biology · 2014Article
- Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis.The Journal of cell biology · 2014Article
- Centromeric heterochromatin: the primordial segregation machine.Annual review of genetics · 2014Review
- Global 'bootprinting' reveals the elastic architecture of the yeast TFIIIB-TFIIIC transcription complex in vivo.Nucleic acids research · 2013Article
- Silencing near tRNA genes is nucleosome-mediated and distinct from boundary element function.Gene · 2013Article
- Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The hierarchy of the 3D genome.Molecular cell · 2013Review
- TFIIIC localizes budding yeast ETC sites to the nuclear periphery.Molecular biology of the cell · 2012Article
- Structural and functional evidence of high specificity of Cbf5 for ACA trinucleotide.RNA (New York, N.Y.) · 2011Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is independent of the orientation of the tRNA gene and does not require direct, steric interference with the binding of either upstream pol II factors or the pol II holoenzyme. A mutant was isolated in which this form of silencing is suppressed. The responsible point mutation affects expression of the Cbf5 protein, a small nucleolar ribonucleoprotein protein required for correct processing of rRNA. Because some early steps in the S. cerevisiae pre-tRNA biosynthetic pathway are nucleolar, we examined whether the CBF5 mutation might affect this localization. Nucleoli were slightly fragmented, and the pre-tRNAs went from their normal, mostly nucleolar location to being dispersed in the nucleoplasm. A possible mechanism for tRNA gene-mediated silencing is suggested in which subnuclear localization of tRNA genes antagonizes transcription of nearby genes by pol II.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.