ArticleProceedings of the National Academy of Sciences of the United States of America2000
Y chromosomal fertility factors kl-2 and kl-3 of Drosophila melanogaster encode dynein heavy chain polypeptides.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis reveals that genes regulated by the Y chromosome in Drosophila melanogaster are preferentially localized to repressive chromatin.Genome biology and evolution · 2013Pooled it
- Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive in Drosophila.Nature communications · 2026Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive inbioRxiv : the preprint server for biology · 2026Article
- Defective splicing of Y-chromosome-linked gigantic genes contributes to hybrid male sterility in Drosophila.Molecular biology and evolution · 2026Article
- Male sterility in Drosophila hybrids revealed by a multi-generational transcriptomic analysis of genes and transposable elements in testes.BMC biology · 2026Article
- Strong bias in long-read sequencing prevents assembly ofGenome research · 2026Article
- Male sterility in Drosophila mojavensis × D. arizonae hybrids is not caused by gene movements in the Y chromosome.Evolution; international journal of organic evolution · 2025Article
- Telomere-to-telomere genome assembly uncovers Wolbachia-driven recurrent male bottleneck effect and selection in a sawfly.Communications biology · 2025Article
- Co-transcriptional splicing facilitates transcription of gigantic genes.PLoS genetics · 2024Article
- The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract.Communications biology · 2024Article
- Paralog transcriptional differentiation in the D. melanogaster-specific gene family Sdic across populations and spermatogenesis stages.Communications biology · 2023Article
- Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility.Nature communications · 2023Article
- Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq ofeLife · 2023Article
- Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in Situ Hybridization in Whole Mount Drosophila Testes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ribosomal DNA Instability as a Potential Cause of Karyotype Evolution.Molecular biology and evolution · 2022Article
- Sex-limited chromosomes and non-reproductive traits.BMC biology · 2022Review
- Review
- Antimicrobial Properties of a Peptide Derived from the Male Fertility Factor kl2 Protein ofCurrent issues in molecular biology · 2022Article
- New Genes in theGenes · 2021Article
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular identity and function of the Drosophila melanogaster Y-linked fertility factors have long eluded researchers. Although the D. melanogaster genome sequence was recently completed, the fertility factors still were not identified, in part because of low cloning efficiency of heterochromatic Y sequences. Here we report a method for iterative blast searching to assemble heterochromatic genes from shotgun assemblies, and we successfully identify kl-2 and kl-3 as 1beta- and gamma-dynein heavy chains, respectively. Our conclusions are supported by formal genetics with X-Y translocation lines. Reverse transcription-PCR was successful in linking together unmapped sequence fragments from the whole-genome shotgun assembly, although some sequences were missing altogether from the shotgun effort and had to be generated de novo. We also found a previously undescribed Y gene, polycystine-related (PRY). The closest paralogs of kl-2, kl-3, and PRY (and also of kl-5) are autosomal and not X-linked, suggesting that the evolution of the Drosophila Y chromosome has been driven by an accumulation of male-related genes arising de novo from the autosomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.