ArticleThe Journal of experimental medicine2000
Identification of a novel prostaglandin f(2alpha) synthase in Trypanosoma brucei.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 104 citations in OpenAlex.
- The intersection of hostMicrobiology and molecular biology reviews : MMBR · 2024Review
- Parasitic infections: A new frontier for PGDCurrent research in immunology · 2024Review
- Fatty Acid Composition and Metabolism inInternational journal of molecular sciences · 2023Review
- New Insights into the Role of thePathogens (Basel, Switzerland) · 2023Article
- Trypanosoma brucei: Metabolomics for analysis of cellular metabolism and drug discovery.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- Lipid droplets of protozoan parasites: survival and pathogenicity.Memorias do Instituto Oswaldo Cruz · 2022Article
- Sleeping Sickness Disrupts the Sleep-Regulating Adenosine System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Review
- Leishmania braziliensis prostaglandin FParasites & vectors · 2020Article
- Eicosanoid production byVirulence · 2019Review
- Co-opting oxylipin signals in microbial disease.Cellular microbiology · 2019Review
- A role for trypanosomatid aldo-keto reductases in methylglyoxal, prostaglandin and isoprostane metabolism.The Biochemical journal · 2018Article
- Trypanosoma cruzi Produces the Specialized Proresolving Mediators Resolvin D1, Resolvin D5, and Resolvin E2.Infection and immunity · 2018Article
- Inflammatory and Pro-resolving Lipids in Trypanosomatid Infections: A Key to Understanding Parasite Control.Frontiers in microbiology · 2018Review
- Old Yellow Enzyme fromFrontiers in immunology · 2018Article
- Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study.Scientific reports · 2016Article
- Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient.PLoS neglected tropical diseases · 2015Article
- Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence.The Journal of infectious diseases · 2014Article
- Insect stage-specific receptor adenylate cyclases are localized to distinct subdomains of the Trypanosoma brucei Flagellar membrane.Eukaryotic cell · 2014Article
- Cyclical appearance of African trypanosomes in the cerebrospinal fluid: new insights in how trypanosomes enter the CNS.PloS one · 2014Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Trypanosoma cause African trypanosomiasis in humans and animals in Africa. Infection of mammals by African trypanosomes is characterized by an upregulation of prostaglandin (PG) production in the plasma and cerebrospinal fluid. These metabolites of arachidonic acid (AA) may, in part, be responsible for symptoms such as fever, headache, immunosuppression, deep muscle hyperaesthesia, miscarriage, ovarian dysfunction, sleepiness, and other symptoms observed in patients with chronic African trypanosomiasis. Here, we show that the protozoan parasite T. brucei is involved in PG production and that it produces PGs enzymatically from AA and its metabolite, PGH(2). Among all PGs synthesized, PGF(2alpha) was the major prostanoid produced by trypanosome lysates. We have purified a novel T. brucei PGF(2alpha) synthase (TbPGFS) and cloned its cDNA. Phylogenetic analysis and molecular properties revealed that TbPGFS is completely distinct from mammalian PGF synthases. We also found that TbPGFS mRNA expression and TbPGFS activity were high in the early logarithmic growth phase and low during the stationary phase. The characterization of TbPGFS and its gene in T. brucei provides a basis for the molecular analysis of the role of parasite-derived PGF(2alpha) in the physiology of the parasite and the pathogenesis of African trypanosomiasis.
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