ArticleGenetics2000
Self-compatible B mutants in coprinus with altered pheromone-receptor specificities.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Article
- Discovery and Functional Study of a Novel Genomic Locus Homologous toMycobiology · 2021Article
- Article
- Activation of the Mating Pheromone Response Pathway ofMycobiology · 2018Article
- Fungal Sex: The Basidiomycota.Microbiology spectrum · 2017Review
- Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusG3 (Bethesda, Md.) · 2016Article
- Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea.PloS one · 2015Article
- Identification and functional analysis of pheromone and receptor genes in the B3 mating locus of Pleurotus eryngii.PloS one · 2014Article
- Interspecific sex in grass smuts and the genetic diversity of their pheromone-receptor system.PLoS genetics · 2011Article
- Evidence for maintenance of sex determinants but not of sexual stages in red yeasts, a group of early diverged basidiomycetes.BMC evolutionary biology · 2011Article
- Fungal mating pheromones: choreographing the dating game.Fungal genetics and biology : FG & B · 2011Review
- Sex in fungi.Annual review of genetics · 2011Review
- A constitutively active GPCR governs morphogenic transitions in Cryptococcus neoformans.The EMBO journal · 2009Article
- Orchestration of sexual reproduction and virulence by the fungal mating-type locus.Current opinion in microbiology · 2008Review
- Article
- Article
- The evolution of non-reciprocal nuclear exchange in mushrooms as a consequence of genomic conflict.Proceedings. Biological sciences · 2004Article
- Evolution of multispecific mating-type alleles for pheromone perception in the homobasidiomycete fungi.Current genetics · 2003Article
- Pheromones stimulate mating and differentiation via paracrine and autocrine signaling in Cryptococcus neoformans.Eukaryotic cell · 2002Article
- Changes in mate recognition through alterations of pheromones and receptors in the multisexual mushroom fungus Schizophyllum commune.Genetics · 2001Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A successful mating in the mushroom Coprinus cinereus brings together a compatible complement of pheromones and G-protein-coupled receptors encoded by multiallelic genes at the B mating-type locus. Rare B gene mutations lead to constitutive activation of B-regulated development without the need for mating. Here we characterize a mutation that arose in the B6 locus and show that it generates a mutant receptor with a single amino acid substitution (R96H) at the intracellular end of transmembrane domain III. Using a heterologous yeast assay and synthetic pheromones we show that the mutation does not make the receptor constitutively active but permits it to respond inappropriately to a normally incompatible pheromone encoded within the same B6 locus. Parallel experiments carried out in Coprinus showed that a F67W substitution in this same pheromone enabled it to activate the normally incompatible wild-type receptor. Together, our experiments show that a single amino acid replacement in either pheromone or receptor can deregulate the specificity of ligand-receptor recognition and confer a self-compatible B phenotype. In addition, we use the yeast assay to demonstrate that different receptors and pheromones found at a single B locus belong to discrete subfamilies within which receptor activation cannot normally occur.
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