ArticleGenetics2000
Fission yeast Ras1 effector Scd1 interacts with the spindle and affects its proper formation.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Genome Sequencing ofJournal of fungi (Basel, Switzerland) · 2024Article
- Functional and structural insights into RAS effector proteins.Molecular cell · 2024Review
- Review
- Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis.Molecular biology of the cell · 2015Article
- Ras1 acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans.PLoS genetics · 2013Article
- Ras signaling in yeast.Genes & cancer · 2011Article
- Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles.Cell motility and the cytoskeleton · 2008Article
- Ras GTPase-activating protein gap1 of the homobasidiomycete Schizophyllum commune regulates hyphal growth orientation and sexual development.Eukaryotic cell · 2006Article
- Article
- Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.Genetics · 2003Article
- Gef1p and Scd1p, the Two GDP-GTP exchange factors for Cdc42p, form a ring structure that shrinks during cytokinesis in Schizosaccharomyces pombe.Molecular biology of the cell · 2003Article
- Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates polarity in Schizosaccharomyces pombe.Molecular biology of the cell · 2003Article
- Two ras pathways in fission yeast are differentially regulated by two ras guanine nucleotide exchange factors.Molecular and cellular biology · 2002Article
- Yin6, a fission yeast Int6 homolog, complexes with Moe1 and plays a role in chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- A conserved interaction between Moe1 and Mal3 is important for proper spindle formation in Schizosaccharomyces pombe.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ras1 GTPase is the Schizosaccharomyces pombe homolog of the mammalian Ha-Ras proto-oncoprotein. Ras1 interacts with Scd1 (aka Ral1), a presumptive guanine nucleotide exchange factor for Cdc42sp, to control organization of the cytoskeleton. In this study, we demonstrated that the scd1 deletion (scd1Delta) induced hypersensitivity to microtubule destabilizing drugs and instability of the minichromosome. Overexpression of scd1 induced formation of abnormal spindles and chromosome missegregation. The scd1 deletion worsened the defects of spindle formation in tubulin mutants; by contrast, it did not induce lethality in mutants defective in the spindle pole bodies. These genetic data suggest that Scd1 can interact with tubulin with substantial specificity to affect proper spindle formation and chromosome segregation. Subcellular localization data further illustrated that a GFP-Scd1 fusion protein can associate with the spindle. Finally, we showed that unlike ras1Delta and scd1Delta, byr2Delta (affecting the Ras1 effector for mating) is not synthetically lethal with the tubulin mutations. These data collectively suggest that the Ras1 pathway can impinge upon microtubules through Scd1, but not Byr2, to affect proper spindle formation and chromosome segregation.
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