ArticleThe Journal of cell biology2000
Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed.
- Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols.Nature communications · 2022Article
- Potassium permanganate is an excellent alternative to osmium tetroxide in freeze-substitution.Histochemistry and cell biology · 2022Article
- MAR1 links membrane adhesion to membrane merger during cell-cell fusion in Chlamydomonas.Developmental cell · 2021Article
- Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion.The Journal of cell biology · 2021Article
- Plasma Membrane Fusion Is Specifically Impacted by the Molecular Structure of Membrane Sterols During Vegetative Development ofGenetics · 2020Article
- Overview ofIndian journal of microbiology · 2020Review
- The molecular foundations of zygosis.Cellular and molecular life sciences : CMLS · 2020Review
- Article
- How cells fuse.The Journal of cell biology · 2019Review
- The ancient claudin Dni2 facilitates yeast cell fusion by compartmentalizing Dni1 into a membrane subdomain.Cellular and molecular life sciences : CMLS · 2018Article
- Genomic and Genetic Insights Into a Cosmopolitan Fungus,Frontiers in microbiology · 2018Article
- Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion.The Journal of cell biology · 2017Article
- PRM1 and KAR5 function in cell-cell fusion and karyogamy to drive distinct bisexual and unisexual cycles in the Cryptococcus pathogenic species complex.PLoS genetics · 2017Article
- Automated analysis of high-content microscopy data with deep learning.Molecular systems biology · 2017Article
- Thigmo Responses: The Fungal Sense of Touch.Microbiology spectrum · 2017Review
- A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.PLoS genetics · 2017Article
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Article
25 more citing papers are in PubMed but not listed here.
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell fusion occurs throughout development, from fertilization to organogenesis. The molecular mechanisms driving plasma membrane fusion in these processes remain unknown. While yeast mating offers an excellent model system in which to study cell fusion, all genes previously shown to regulate the process act at or before cell wall breakdown; i.e., well before the two plasma membranes have come in contact. Using a new strategy in which genomic data is used to predict which genes may possess a given function, we identified PRM1, a gene that is selectively expressed during mating and that encodes a multispanning transmembrane protein. Prm1p localizes to sites of cell-cell contact where fusion occurs. In matings between Deltaprm1 mutants, a large fraction of cells initiate zygote formation and degrade the cell wall separating mating partners but then fail to fuse. Electron microscopic analysis reveals that the two plasma membranes in these mating pairs are tightly apposed, remaining separated only by a uniform gap of approximately 8 nm. Thus, the phenotype of Deltaprm1 mutants defines a new step in the mating reaction in which membranes are juxtaposed, possibly through a defined adherence junction, yet remain unfused. This phenotype suggests a role for Prm1p in plasma membrane fusion.
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