ArticleThe Journal of cell biology2000
Evidence that beta3 integrin-induced Rac activation involves the calpain-dependent formation of integrin clusters that are distinct from the focal complexes and focal adhesions that form as Rac and RhoA become active.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
38 citing papers in PubMed, 100 citations in OpenAlex.
- Rare variants inHGG advances · 2023Article
- Calpains, the proteases of two faces controlling the epithelial homeostasis in mammary gland.Frontiers in cell and developmental biology · 2023Review
- Binding of the Andes Virus Nucleocapsid Protein to RhoGDI Induces the Release and Activation of the Permeability Factor RhoA.Journal of virology · 2021Article
- How Cells Communicate with Each Other in the Tumor Microenvironment: Suggestions to Design Novel Therapeutic Strategies in Cancer Disease.International journal of molecular sciences · 2021Review
- Microarray Expression Profile and Analysis of Circular RNA Regulatory Network in Pulmonary Thromboembolism.International journal of general medicine · 2021Article
- Recent Progress of RGD Modified Liposomes as Multistage Rocket Against Cancer.Frontiers in pharmacology · 2021Review
- Cancer cells increase endothelial cell tube formation and survival by activating the PI3K/Akt signalling pathway.Journal of experimental & clinical cancer research : CR · 2017Article
- The Autophagy Machinery: A New Player in Chemotactic Cell Migration.Frontiers in neuroscience · 2017Review
- Epistemology of the origin of cancer: a new paradigm.BMC cancer · 2014Article
- Targeted gene inactivation reveals a functional role of calpain-1 in platelet spreading.Journal of thrombosis and haemostasis : JTH · 2012Article
- Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.Molecular biology of the cell · 2012Article
- Comparative proteomic analysis of a cytosolic fraction from β3 integrin-deficient cells.Cancer genomics & proteomics · 2012Article
- Calpain-6, a target molecule of glucocorticoids, regulates osteoclastic bone resorption via cytoskeletal organization and microtubule acetylation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2011Article
- Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness.Microvascular research · 2010Article
- Redox control of vascular smooth muscle migration.Antioxidants & redox signaling · 2010Review
- Modulation of integrin antagonist signaling by ligand binding of the heparin-binding domain of vitronectin to the alphaVbeta3 integrin.Journal of cellular biochemistry · 2008Article
- Calpain 1 and 2 are required for RNA replication of echovirus 1.Journal of virology · 2008Article
- Modeling cancer progression via pathway dependencies.PLoS computational biology · 2008Article
- Actin cytoskeletal mediators of motility and invasion amplified and overexpressed in head and neck cancer.Clinical & experimental metastasis · 2008Review
- Role of phospholipase Cgamma1 in cell spreading requires association with a beta-Pix/GIT1-containing complex, leading to activation of Cdc42 and Rac1.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
Interaction of integrins with the extracellular matrix leads to transmission of signals, cytoskeletal reorganizations, and changes in cell behavior. While many signaling molecules are known to be activated within Rac-induced focal complexes or Rho-induced focal adhesions, the way in which integrin-mediated adhesion leads to activation of Rac and Rho is not known. In the present study, we identified clusters of integrin that formed upstream of Rac activation. These clusters contained a Rac-binding protein(s) and appeared to be involved in Rac activation. The integrin clusters contained calpain and calpain-cleaved beta3 integrin, while the focal complexes and focal adhesions that formed once Rac and Rho were activated did not. Moreover, the integrin clusters were dependent on calpain for their formation. In contrast, while Rac- and Rho-GTPases were dependent on calpain for their activation, formation of focal complexes and focal adhesions by constitutively active Rac or Rho, respectively, occurred even when calpain inhibitors were present. Taken together, these data are consistent with a model in which integrin-induced Rac activation requires the formation of integrin clusters. The clusters form in a calpain-dependent manner, contain calpain, calpain-cleaved integrin, and a Rac binding protein(s). Once Rac is activated, other integrin signaling complexes are formed by a calpain-independent mechanism(s).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.