ArticleJournal of clinical microbiology2000
Improved version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for hepatitis C virus RNA: calibration to international units, enhanced genotype reactivity, and performance characteristics.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 153 citations in OpenAlex.
- Comparison of conventional PCR with real-time PCR and branched DNA-based assays for hepatitis C virus RNA quantification and clinical significance for genotypes 1 to 5.Journal of clinical microbiology · 2006Trial
- Dynamic range of hepatitis C virus RNA quantification with the Cobas Ampliprep-Cobas Amplicor HCV Monitor v2.0 assay.Journal of clinical microbiology · 2005Trial
- Assessment, by transcription-mediated amplification, of virologic response in patients with chronic hepatitis C virus treated with peginterferon alpha-2a.Journal of clinical microbiology · 2001Trial
- Article
- Red complex periodontal pathogens are risk factors for liver cirrhosis.Biomedical reports · 2019Article
- HCV Detection, Discrimination, and Genotyping Technologies.Sensors (Basel, Switzerland) · 2018Review
- The future of viral hepatitis testing: innovations in testing technologies and approaches.BMC infectious diseases · 2017Review
- Successful Treatment of Hepatitis C Virus-associated Oral Lichen Planus by Interferon-free Therapy with Direct-acting Antivirals.Clinical and translational gastroenterology · 2016Article
- Update on hepatitis B and C virus diagnosis.World journal of virology · 2015Review
- Current molecular methods for the detection of hepatitis C virus in high risk group population: A systematic review.World journal of virology · 2015Review
- Association of Periodontal Diseases and Liver Fibrosis in Patients With HCV and/or HBV infection.Hepatitis monthly · 2014Article
- Is HCV core antigen a reliable marker of viral load? An evaluation of HCV core antigen automated immunoassay.Annals of gastroenterology · 2013Article
- Effect of branched-chain amino acid-enriched nutritional supplementation on interferon therapy in Japanese patients with chronic hepatitis C virus infection: a retrospective study.Virology journal · 2012Article
- Candidiasis and other oral mucosal lesions during and after interferon therapy for HCV-related chronic liver diseases.BMC gastroenterology · 2012Article
- Performance of the novel Qiagen artus QS-RGQ viral load assays compared to that of the Abbott RealTime system with genetically diversified HIV and hepatitis C Virus plasma specimens.Journal of clinical microbiology · 2012Article
- A retrospective case-control study of hepatitis C virus infection and oral lichen planus in Japan: association study with mutations in the core and NS5A region of hepatitis C virus.BMC gastroenterology · 2012Article
- Dental problems delaying the initiation of interferon therapy for HCV-infected patients.Virology journal · 2010Article
- Excellent superiority and specificity of COBAS TaqMan HCV assay in an early viral kinetic change during pegylated interferon alpha-2b plus ribavirin treatment.BMC gastroenterology · 2010Article
- Prion protein expression and processing in human mononuclear cells: the impact of the codon 129 prion gene polymorphism.PloS one · 2009Article
- Diagnosing norovirus-associated infectious intestinal disease using viral load.BMC infectious diseases · 2009Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Version 2.0 qualitative and quantitative AMPLICOR reverse transcription-PCR tests for HCV were designed to improve on the performance of first version of the hepatitis C virus (HCV) tests. The new tests were calibrated in international units, the new commonly accepted standard unit of measurement for HCV RNA. The sensitivity of the qualitative tests was enhanced by modifying the specimen processing procedure to achieve a limit of detection 50 IU/ml. The limit of detection for the quantitative tests was 600 IU/ml. Modifications to the amplification reaction mixture and thermal cycling conditions enabled all genotypes to be amplified with similar efficiency. The quantitative tests exhibited a linear range extending from 500 to 500,000 IU/ml and excellent reproducibility, with coefficients of variation ranging from 18 to 39%, within the linear range. These data indicate that the version 2. 0 AMPLICOR HCV tests will improve diagnosis of HCV infection and will yield more-accurate titers for prognosis and for monitoring therapeutic efficacy, particularly at low viral loads. Furthermore, it will be possible to compare the performance characteristics and viral load measurements of AMPLICOR tests to those of other tests that adopt the international unit as the standard of measurement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.