ArticleJournal of clinical microbiology2000
Characterization of a Borrelia burgdorferi VlsE invariable region useful in canine Lyme disease serodiagnosis by enzyme-linked immunosorbent assay.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 91 citations in OpenAlex.
- Pre-treatment and post-treatment assessment of the C(6) test in patients with persistent symptoms and a history of Lyme borreliosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Trial
- Seropositivity of main vector-borne pathogens in dogs across Europe.Parasites & vectors · 2022Article
- Evaluation of 2 ELISAs to determineJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Detection of antibodies to decorin-binding protein A (DbpA) and DbpB after infection of dogs withJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- A fluorescent plasmonic biochip assay for multiplex screening of diagnostic serum antibody targets in human Lyme disease.PloS one · 2020Article
- A Suspected Case of Lyme Borreliosis in a Dog from Belgium.Case reports in veterinary medicine · 2019Article
- Zoonotic Anaplasma phagocytophilum, Ehrlichia canis, Dirofilaria immitis, Borrelia burgdorferi, and spotted fever group rickettsiae (SFGR) in different types of dogs.Parasitology research · 2018Article
- Canine and human infection with Borrelia burgdorferi in the New York City metropolitan area.Parasites & vectors · 2018Article
- Article
- Lyme disease risk in dogs in New Brunswick.The Canadian veterinary journal = La revue veterinaire canadienne · 2016Article
- Tick-borne Diseases (Borreliosis, Anaplasmosis, Babesiosis) in German and Austrian Dogs: Status quo and Review of Distribution, Transmission, Clinical Findings, Diagnostics and Prophylaxis.Parasitology research · 2015Article
- Seroprevalence of equine granulocytic anaplasmosis and lyme borreliosis in Canada as determined by a point-of-care enzyme-linked immunosorbent assay (ELISA).The Canadian veterinary journal = La revue veterinaire canadienne · 2015Article
- Bacterial heterogeneity is a requirement for host superinfection by the Lyme disease spirochete.Infection and immunity · 2014Article
- Diversity of antibody responses to Borrelia burgdorferi in experimentally infected beagle dogs.Clinical and vaccine immunology : CVI · 2014Article
- Serum antibodies to Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti in recaptured white-footed mice.Journal of wildlife diseases · 2013Article
- Article
- Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 antigens as markers for early and late infection in dogs.Clinical and vaccine immunology : CVI · 2012Article
- Serum antibodies to whole-cell and recombinant antigens of Borrelia burgdorferi in cottontail rabbits.Journal of wildlife diseases · 2012Article
- Borrelia burgdorferi sensu lato species in Europe induce diverse immune responses against C6 peptides in infected mice.Clinical and vaccine immunology : CVI · 2009Article
- A suspected case of Lyme borreliosis in a hunting dog in Korea.Journal of veterinary science · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Sera collected from dogs experimentally infected with Borrelia burgdorferi by tick inoculation were analyzed for an antibody response to each of the six invariable regions (IRs; i.e., IR(1) to IR(6)) of VlsE, the variable surface antigen of B. burgdorferi. Six synthetic peptides (C(1) to C(6)), which reproduced the six IR sequences were used as peptide-based, enzyme-linked immunosorbent assay (ELISA) antigens. Two IRs, IR(2) and IR(6), were found to be immunodominant. Studies with serially collected serum samples from experimentally infected dogs revealed that the antibody response to IR(6) appears earlier and is stronger than that to IR(2). Thus, the IR(6) sequence alone appeared to be sufficient for serodiagnosis. When C(6) alone was used as antigen, the peptide-based ELISA was positive in 7 of 23 dogs (30%) as early as 3 weeks postinfection. All dogs (n = 33) became strongly positive 1 or 2 weeks later, and this response persisted for the entire study, which lasted for 69 weeks. Of 55 sera submitted by veterinarians from dogs suspected of having Lyme disease, 19 were also positive by the C(6) ELISA, compared to 20 positives detected by immunoblot analysis using cultured B. burgdorferi lysates as antigen. The sensitivity of using C(2) and C(6) together for detecting specific antibody in both experimentally infected and clinically diagnosed dogs was not better than sensitivity with C(6) alone, confirming that C(6) suffices as a diagnostic probe. Moreover, the C(6) ELISA yielded 100% specificity with serum samples collected from 70 healthy dogs, 14 dogs with infections other than B. burgdorferi, and 15 animals vaccinated with either outer surface protein A, whole-spirochete vaccines, or the common puppy-vaccines. Therefore, this C(6) ELISA was both sensitive and specific for the serodiagnosis of canine Lyme disease and could be used with vaccinated dogs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.