ArticleJournal of clinical microbiology2000
PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 154 citations in OpenAlex.
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- A Low-Cost, 3D-Printed Biosensor for Rapid Detection ofSensors (Basel, Switzerland) · 2022Article
- Molecular Detection of Non-O157 Shiga Toxin-ProducingMicroorganisms · 2022Article
- A Phage Receptor-Binding Protein as a Promising Tool for the Detection ofFrontiers in microbiology · 2022Article
- Giant Amoebic Liver Abscess: A Rare Diagnosis in a Rural Setting of Sub-Saharan Africa.Journal of parasitology research · 2021Article
- Comparison of five assays for DNA extraction from bacterial cells in human faecal samples.Journal of applied microbiology · 2020Article
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- Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device.Biosensors & bioelectronics · 2019Article
- Epidemiology of Shiga Toxin-ProducingToxins · 2019Article
- A novel ready-to-use dry-reagent polymerase chain reaction for detection ofThe Indian journal of medical research · 2019Article
- Whatman Protein Saver Cards for Storage and Detection of Parasitic Enteropathogens.The American journal of tropical medicine and hygiene · 2018Article
- CPHLN recommendations for the laboratory detection of Shiga toxin-producingCanada communicable disease report = Releve des maladies transmissibles au Canada · 2018Article
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- Comparison of two methods for detection ofIranian journal of microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid identification of Escherichia coli O157:H7 is important for patient management and for prompt epidemiological investigations. We evaluated one in-house method and three commercially available kits for their ability to extract E. coli O157:H7 DNA directly from stool specimens for PCR. Of the 153 stool specimens tested, 107 were culture positive and 46 were culture negative. The sensitivities and specificities of the in-house enrichment method, IsoQuick kit, NucliSens kit, and QIAamp kit were comparable, as follows: 83 and 98%, 85 and 100%, 74 and 98%, and 86 and 100%, respectively. False-negative PCR results may be due to the presence of either inherent inhibitors or small numbers of organisms. The presence of large amounts of bacteria relative to the amount of the E. coli O157:H7 target may result in the lower sensitivities of the assays. All commercial kits were rapid and easy to use, although DNA extracted with the QIAamp kit did not require further dilution of the DNA template prior to PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.