ArticleJournal of clinical microbiology2000
Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- Article
- Refining malaria diagnosis in high-transmission areas: a dual-approach with rapid diagnostic tests (RDTs) and dbPCR-NALFIA.Malaria journal · 2025Article
- Clarifying the terms parasitemia, parasite density, and parasite count.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2023Article
- Article
- Laboratory evaluation of the miniature direct-on-blood PCR nucleic acid lateral flow immunoassay (mini-dbPCR-NALFIA), a simplified molecular diagnostic test for Plasmodium.Malaria journal · 2023Article
- Urine and Saliva: Relevant Specimens for Malaria Diagnosis?Diagnostics (Basel, Switzerland) · 2022Review
- Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study.The Korean journal of parasitology · 2022Article
- Magneto-optical diagnosis of symptomatic malaria in Papua New Guinea.Nature communications · 2021Article
- Transfusion-Transmitted Malaria: A Systematic Review and Meta-analysis.Open forum infectious diseases · 2019Article
- Diagnostic tools in childhood malaria.Parasites & vectors · 2018Review
- A sensitive species-specific reverse transcription real-time PCR method for detection ofParasite epidemiology and control · 2017Article
- Spatial repellents and malaria transmission in an endemic area of Cambodia with high mosquito net usage.MalariaWorld journal · 2017Article
- Assessment of submicroscopic infections and gametocyte carriage of Plasmodium falciparum during peak malaria transmission season in a community-based cross-sectional survey in western Kenya, 2012.Malaria journal · 2016Article
- Article
- Malaria Diagnosis Across the International Centers of Excellence for Malaria Research: Platforms, Performance, and Standardization.The American journal of tropical medicine and hygiene · 2015Review
- Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets.PLoS medicine · 2015Article
- Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.The Journal of antimicrobial chemotherapy · 2015Review
- Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro.PloS one · 2015Article
- Asymptomatic malaria infections: detectability, transmissibility and public health relevance.Nature reviews. Microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified in vitro RNA as a competitor in a single-tube NASBA reaction. Parasite densities ranging from 10 to 10(8) Plasmodium falciparum parasites per ml could be demonstrated and quantified in whole blood. This is approximately 1,000 times more sensitive than conventional microscopy analysis of thick blood smears. Comparison of the parasite densities obtained by microscopy and QT-NASBA with 120 blood samples from Kenyan patients with clinical malaria revealed that for 112 of 120 (93%) of the samples results were within a 1-log difference. QT-NASBA may be especially useful for the detection of low parasite levels in patients with early-stage malaria and for the monitoring of the efficacy of drug treatment.
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