ArticleJournal of clinical microbiology2000
Evaluation of fluorescence-based amplified fragment length polymorphism analysis for molecular typing in hospital epidemiology: comparison with pulsed-field gel electrophoresis for typing strains of vancomycin-resistant Enterococcus faecium.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- Newer diagnostic tests for bacterial diseases.Indian journal of pediatrics · 2007Review
- Identification of Clostridium species and DNA fingerprinting of Clostridium perfringens by amplified fragment length polymorphism analysis.Journal of clinical microbiology · 2006Article
- Efficient DNA fingerprinting of Clostridium botulinum types A, B, E, and F by amplified fragment length polymorphism analysis.Applied and environmental microbiology · 2005Article
- Detection of enterococcal surface protein gene (esp) and amplified fragment length polymorphism typing of glycopeptide-resistant Enterococcus faecium during its emergence in a Greek intensive care unit.Journal of clinical microbiology · 2003Article
- Acquisition and duration of vancomycin-resistant enterococcal carriage in relation to strain type.Journal of clinical microbiology · 2003Article
- Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium.Journal of clinical microbiology · 2003Article
- First characterization of a cluster of VanA-type glycopeptide-resistant Enterococcus faecium, Colombia.Emerging infectious diseases · 2002Article
- Fluorescent amplified fragment length polymorphism probabilistic database for identification of bacterial isolates from urinary tract infections.Journal of clinical microbiology · 2002Article
- Multilocus sequence typing scheme for Enterococcus faecium.Journal of clinical microbiology · 2002Article
- Automated ribotyping of vancomycin-resistant Enterococcus faecium isolates.Journal of clinical microbiology · 2002Article
- Vancomycin-resistant Enterococcus faecium (VREF) from Norwegian poultry cluster with VREF from poultry from the United Kingdom and The Netherlands in an amplified fragment length polymorphism genogroup.Applied and environmental microbiology · 2002Article
- Healthcare-associated vancomycin resistant Enterococcus faecium infections in the Mansoura University Hospitals intensive care units, Egypt.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence-based amplified fragment length polymorphism (fbAFLP) is a novel assay based on the fluorescent analysis of an amplified subset of restriction fragments. The fbAFLP assay involves the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The ligation of adapters with primer-specific sites coupled with primers containing selective nucleotides allowed the full potential of PCR to be realized while maintaining the advantages of restriction endonuclease analysis. Fluorescence-based fragment analysis with polyacrylamide gel electrophoresis provides the accurate band sizing required for homology assessment. The large number of phylogenetically informative characters obtained by fbAFLP is well suited for cluster analysis and database development. The method demonstrated excellent reproducibility and ease of performance and interpretation. We typed 30 epidemiologically well-characterized isolates of vancomycin-resistant enterococci from an outbreak in a university hospital by fbAFLP. Clustering of fbAFLP data matched epidemiological, microbiological, and pulsed-field gel electrophoresis data. This study demonstrates the unprecedented utility of fbAFLP for epidemiological investigation. Future developments in standardization and automation will set fbAFLP as the "gold standard" for molecular typing in epidemiology.
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