ArticleJournal of clinical microbiology2000
Real-time quantitative PCR for human herpesvirus 6 DNA.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 140 citations in OpenAlex.
- Beyond Infection: The Interplay of Salivary Human Herpesvirus 6, Stress, and Host Factors in Major Depressive Disorder.Viruses · 2026Article
- Analysis Of Salivary Herpesviruses Reveals Associations Between HHV-6 And Long COVID Severity.medRxiv : the preprint server for health sciences · 2026Article
- Frequency and clinical impact of viraemia in paediatric patients undergoing therapy for cancer.Scientific reports · 2024Observational
- Analysis of Suspected Measles Cases with Discrepant Measles-Specific IgM and rRT-PCR Test Results, Japan.Emerging infectious diseases · 2024Article
- A robust host-response-based signature distinguishes bacterial and viral infections across diverse global populations.Cell reports. Medicine · 2022Article
- Herpes Infections in Suspected Cases of Yellow Fever in the Democratic Republic of the Congo.Medicina (Kaunas, Lithuania) · 2021Article
- Successful treatment and FDG-PET/CT monitoring of HHV-6 encephalitis in a non-neutropenic patient: case report and literature review.Journal of neurovirology · 2017Article
- Subclinical herpesvirus shedding among HIV-1-infected men on antiretroviral therapy.AIDS (London, England) · 2017Article
- Aetiology and Outcomes of Suspected Infections of the Central Nervous System in Children in Mbarara, Uganda.Scientific reports · 2017Article
- Clinical features and seasonality of parechovirus infection in an Asian subtropical city, Hong Kong.PloS one · 2017Article
- Optimisation of a quantitative polymerase chain reaction-based strategy for the detection and quantification of human herpesvirus 6 DNA in patients undergoing allogeneic haematopoietic stem cell transplantation.Memorias do Instituto Oswaldo Cruz · 2015Article
- Detection of Herpesviridae in whole blood by multiplex PCR DNA-based microarray analysis after hematopoietic stem cell transplantation.Journal of clinical microbiology · 2014Article
- Identification of the platelet-derived chemokine CXCL4/PF-4 as a broad-spectrum HIV-1 inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections.Journal of medical virology · 2012Article
- Article
- Myocardial inflammation in Duchenne Muscular Dystrophy as a precipitating factor for heart failure: a prospective study.BMC neurology · 2010Article
- Recent advancements for the evaluation of anti-viral activities of natural products.New biotechnology · 2009Review
- Multicenter comparison of PCR assays for detection of human herpesvirus 6 DNA in serum.Journal of clinical microbiology · 2008Article
- Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: development and methodology.Journal of clinical microbiology · 2008Article
- Human herpesvirus 6A accelerates AIDS progression in macaques.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnosis of human herpesvirus 6 (HHV-6) infection represents a complex issue because the most widely used diagnostic tools, such as immunoglobulin G antibody titer determination and qualitative DNA PCR with blood cells, are unable to distinguish between latent (clinically silent) and active (often clinically relevant) infection. We have developed a new, highly sensitive, quantitative PCR assay for the accurate measurement of HHV-6 DNA in tissue-derived cell suspensions and body fluids. The test uses a 5' nuclease, fluorogenic assay combined with real-time detection of PCR amplification products with the ABI PRISM 7700 sequence detector system. The sensitivity of this method is equal to the sensitivity of a nested PCR protocol (lower detection limit, 1 viral genome equivalent/test) for both the A and the B HHV-6 subgroups and shows a wider dynamic range of detection (from 1 to 10(6) viral genome equivalents/test) and a higher degree of accuracy, repeatability, and reproducibility compared to those of a standard quantitative-competitive PCR assay developed with the same reference DNA molecule. The novel technique is versatile, showing the same sensitivity and dynamic range with viral DNA extracted from different fluids (i.e., culture medium or plasma) or from tissue-derived cell suspensions. Furthermore, by virtue of its high-throughput format, this method is well suited for large epidemiological surveys.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.