ArticleJournal of clinical microbiology2000
Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Magnetic Janus origami robot for cross-scale droplet omni-manipulation.Nature communications · 2023Article
- Viral Loads in Ocular Fluids of Acute Retinal Necrosis Eyes Infected by Varicella-Zoster Virus Treated with Intravenous Acyclovir Treatment.Journal of clinical medicine · 2020Article
- Epidemiology and clinical features of viral anterior uveitis in southern Taiwan-diagnosis with polymerase chain reaction.BMC ophthalmology · 2019Article
- Development and padronization of three multiplex PCRs for the diagnosis of Chlamydia trachomatis, Toxoplasma gondii, herpes simplex viruses 1 and 2, and Cytomegalovirus.Molecular biotechnology · 2013Article
- Impact of herpes simplex virus detection in respiratory specimens of patients with suspected viral pneumonia.Infection · 2010Article
- Congenital cytomegalovirus infection in high-risk Canadian infants: Report of a pilot screening study.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2010Article
- Article
- Current applications and future trends of molecular diagnostics in clinical bacteriology.Analytical and bioanalytical chemistry · 2009Review
- Monitoring of cytomegalovirus reactivation in bone marrow transplant recipients by real-time PCR.Pathology oncology research : POR · 2008Article
- Concurrent genotyping and quantitation of cytomegalovirus gB genotypes in solid-organ-transplant recipients by use of a real-time PCR assay.Journal of clinical microbiology · 2008Article
- Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients.Journal of clinical microbiology · 2008Article
- Prediction of cytomegalovirus (CMV) plasma load from evaluation of CMV whole-blood load in samples from renal transplant recipients.Journal of clinical microbiology · 2008Article
- Kinetics of aqueous flare, intraocular pressure and virus-DNA copies in a patient with cytomegalovirus iridocyclitis without retinitis.International ophthalmology · 2007Article
- Usefulness of Herpes Consensus PCR methodology to routine diagnostic testing for herpesviruses infections in clinical specimens.Virology journal · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Sequence diversity in the glycoprotein B gene complicates real-time PCR assays for detection and quantification of cytomegalovirus.Journal of clinical microbiology · 2005Article
- Errors in published sequences of human cytomegalovirus primers and probes: do we need more quality control?Journal of clinical microbiology · 2005Article
- Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens.Journal of clinical microbiology · 2005Article
- Comparison of a duplex quantitative real-time PCR assay and the COBAS Amplicor CMV Monitor test for detection of cytomegalovirus.Journal of clinical microbiology · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBAS AMPLICOR CMV MONITOR (CACM) assay, and a qualitative in-house PCR assay for the presence of CMV DNA. Samples that were reactive in at least two of the three assays were considered CMV DNA positive (n = 95 [47. 5%]), while samples that were nonreactive in two of the three assays were considered CMV DNA negative (n = 105 [52.5%]). Using the LightCycler assay, CMV DNA was detected in 91 of the 95 CMV DNA-positive human specimens (sensitivity, 95.8%; 95% confidence interval [CI], 89.6 to 98.8) and in 1 of the CMV DNA-negative specimens (specificity, 99%; 95% CI, 94.8 to 99.8). Results of CMV load determination as assessed by both quantitative test systems were correlated (r = 0.73; P < 0.0001; 95% CI, 0.61 to 0.81). Results for undiluted samples containing a high CMV load were more accurate with the LightCycler test than were results obtained with the CACM test, which underestimated the viral load of samples containing high DNA copy numbers. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler technology are favorable for the use of this system in the detection of CMV DNA in clinical specimens.
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