ArticleJournal of clinical microbiology2000
Improved immunodiagnosis of cystic hydatid disease by using a synthetic peptide with higher diagnostic value than that of its parent protein, Echinococcus granulosus antigen B.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- Comparative analysis of the diagnostic performance of six major Echinococcus granulosus antigens assessed in a double-blind, randomized multicenter study.Journal of clinical microbiology · 2005Trial
- Evaluation of the Antigenic Potential of Epitopes Derived FromBioinformatics and biology insights · 2025Article
- A novel diagnostic technique for diagnosis ofOpen veterinary journal · 2024Article
- Comparison of Native Hydatid Cyst Fluid (HCF), Lyophilized HCF, Antigen B (AgB) and Lyophilized AgB (LAgB) Originated fromIranian journal of public health · 2023Article
- Screening, construction, and serological identification of the diagnostic antigen molecule EG-06283 for the diagnosis of Echinococcus granulosus.Parasitology research · 2021Article
- Evaluation of the sensitivity and specificity of GST-tagged recombinant antigens 2B2t, Ag5t and DIPOL in ELISA for the diagnosis and follow up of patients with cystic echinococcosis.PLoS neglected tropical diseases · 2020Article
- A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis.Veterinary world · 2020Article
- Survey of echinococcoses in southeastern Qinghai Province, China, and serodiagnostic insights of recombinant Echinococcus granulosus antigen B isoforms.Parasites & vectors · 2019Article
- A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides.Parasite immunology · 2017Article
- Characterisation of Antigen B Protein Species Present in the Hydatid Cyst Fluid of Echinococcus canadensis G7 Genotype.PLoS neglected tropical diseases · 2017Article
- Immunodiagnosis of human hydatid disease: Where do we stand?World journal of methodology · 2015Review
- Development of an Immunochromatographic Test for Diagnosis of Visceral Leishmaniasis Based on Detection of a Circulating Antigen.PLoS neglected tropical diseases · 2015Article
- Evaluation of purified antigen fraction in the immunodiagnosis of cystic echinococcosis.Parasitology research · 2014Article
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- Differential diagnosis of cystic and alveolar echinococcosis using an immunochromatographic test based on the detection of specific antibodies.Parasitology research · 2013Article
- A review on diagnostic and preventive aspects of cystic echinococcosis and human cysticercosis.Tropical parasitology · 2012Article
- Serological diagnosis of lung cystic hydatid disease using the synthetic p176 peptide.Clinical and vaccine immunology : CVI · 2012Article
- Characterisation of the native lipid moiety of Echinococcus granulosus antigen B.PLoS neglected tropical diseases · 2012Article
- Host-parasite relationship in cystic echinococcosis: an evolving story.Clinical & developmental immunology · 2012Review
- Mass spectrometric analysis of the immunodominant glycan epitope of Echinococcus granulosus antigen Ag5.International journal for parasitology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assays are used for the diagnosis of hydatid disease are still imperfect. The reported diagnostic sensitivity and specificity vary greatly depending on the panel of sera used, the laboratory conducting the assay, and, more critically, the antigen used. To contribute to its standardization, we have recently ranked the diagnostic performances of the major parasite antigens and the available synthetic peptides using a large collection of serum samples. That work showed that antigen B (AgB) possesses the highest diagnostic value among these antigens. In the present work we further dissected its antigenicity by analyzing the reactivity of the same panel of sera against a set of synthetic peptides spanning the sequence of both AgB subunits. The N-terminal extension of these subunits appeared to be immunodominant in human infections. A 38-mer peptide (p176) delineated from the N-terminal extension of the AgB/1 subunit performed in an enzyme-linked immunosorbent assay with a higher diagnostic sensitivity (80%) and specificity (94%) than native AgB, Ag5, or any other peptide antigen tested against this collection of serum samples. In view of its high diagnostic value and its nature as a well-defined reproducible antigen, p176 could conveniently be used as a reference standard antigen in the diagnosis of hydatid disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.