ArticleThe EMBO journal2000
Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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Who cites it
61 citing papers in PubMed, 159 citations in OpenAlex.
- Site-specific programming characterizes dynamic post-translational acetylation of histone H2B lysine 108 in mouse embryonic stem cells.Nature communications · 2026Article
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- Differential effect of histone H3.3 depletion on retroviral repression in embryonic stem cells.Clinical epigenetics · 2023Article
- Functional integration of Cas9 gene into the genome of rhesus monkey: possibility of a new biomedical model?Life medicine · 2023Article
- The sound of silence: Transgene silencing in mammalian cell engineering.Cell systems · 2022Review
- Epigenomic signatures on paralogous genes reveal underappreciated universality of active histone codes adopted across animals.Computational and structural biotechnology journal · 2022Article
- A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma.Cell reports methods · 2021Article
- Article
- Regulation of Expression and Latency in BLV and HTLV.Viruses · 2020Review
- Tyroxine Hydroxylase-Positive Neuronal Cell Population is Increased by Temporal Dioxin Exposure at Early Stage of Differentiation from Human Embryonic Stem Cells.International journal of molecular sciences · 2019Article
- Epigenetic regulation of redox state mediates persistent cardiorespiratory abnormalities after long-term intermittent hypoxia.The Journal of physiology · 2017Article
- Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration.Mobile DNA · 2017Article
- Multi-layered global gene regulation in mouse embryonic stem cells.Cellular and molecular life sciences : CMLS · 2015Review
- Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells.Journal of virology · 2014Article
- Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Retroviral DNA methylation and epigenetic repression are mediated by the antiviral host protein Daxx.Journal of virology · 2013Article
- Silencing of proviruses in embryonic cells: efficiency, stability and chromatin modifications.EMBO reports · 2013Article
- Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site.Nucleic acids research · 2012Article
- Pantropic retroviruses as a transduction tool for sea urchin embryos.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain.Gene therapy · 2012Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence a linked locus control region (LCR) beta-globin reporter gene in transgenic mice. MSCV silencing blocks LCR hypersensitive site formation, and silent transgene chromatin is marked differentially by a histone code composed of abundant linker histone H1, deacetylated H3 and acetylated H4. Retrovirus-transduced embryonic stem (ES) cells are silenced predominantly 3 days post-infection, with a small subset expressing enhanced green fluorescent protein to low levels, and silencing is not relieved in de novo methylase-null [dnmt3a-/-;dnmt3b-/-] ES cells. MSCV and HIV-1 sequences also repress reporter transgene expression in Drosophila, demonstrating establishment of silencing in the absence of de novo and maintenance methylases. These findings provide mechanistic insight into a conserved gene silencing mechanism that is de novo methylase independent and that epigenetically marks retrovirus chromatin with a repressive histone code.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.