ArticleThe EMBO journal2000
Srf(-/-) ES cells display non-cell-autonomous impairment in mesodermal differentiation.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 89 citations in OpenAlex.
- Serum Response Factor Reduces Gene Expression Noise and Confers Cell State Stability.Stem cells (Dayton, Ohio) · 2023Article
- Arp2/3 complex activity is necessary for mouse ESC differentiation, times formative pluripotency, and enables lineage specification.Stem cell reports · 2022Article
- Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells.Redox biology · 2018Article
- Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs.Nature communications · 2018Article
- ThymosinStem cells international · 2017Article
- Endothelial depletion of murine SRF/MRTF provokes intracerebral hemorrhagic stroke.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The developmental potential of iPSCs is greatly influenced by reprogramming factor selection.Cell stem cell · 2014Article
- Article
- Endothelial SRF/MRTF ablation causes vascular disease phenotypes in murine retinae.The Journal of clinical investigation · 2013Article
- An intragenic SRF-dependent regulatory motif directs cardiac-specific microRNA-1-1/133a-2 expression.PloS one · 2013Article
- Specific gene signatures and pathways in mesodermal cells and their derivatives derived from embryonic stem cells.Stem cell reviews and reports · 2012Review
- Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium.BMC developmental biology · 2011Article
- Serum response factor utilizes distinct promoter- and enhancer-based mechanisms to regulate cytoskeletal gene expression in macrophages.Molecular and cellular biology · 2011Article
- Identification of New SRF Binding Sites in Genes Modulated by SRF Over-Expression in Mouse Hearts.Gene regulation and systems biology · 2011Article
- Cardiogenesis: an embryological perspective.Journal of cardiovascular translational research · 2010Review
- PKA-dependent phosphorylation of serum response factor inhibits smooth muscle-specific gene expression.Arteriosclerosis, thrombosis, and vascular biology · 2009Article
- Enhancer of polycomb1 acts on serum response factor to regulate skeletal muscle differentiation.The Journal of biological chemistry · 2009Article
- Downregulation of SRF-FOS-JUNB pathway in fumarate hydratase deficiency and in uterine leiomyomas.Oncogene · 2009Article
- MicroRNA regulation of cell lineages in mouse and human embryonic stem cells.Cell stem cell · 2008Article
- Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning.Genome biology · 2007Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The serum response factor (SRF) transcription factor is essential for murine embryogenesis. SRF+(-/-) embryos stop developing at the onset of gastrulation, lacking detectable mesoderm. This developmental defect may reflect cell-autonomous impairment of SRF(-/-) embryonic cells in mesoderm formation. Alternatively, it may be caused by a non-cell-autonomous defect superimposed upon inappropriate provision of mesoderm-inducing signals to primitive ectodermal cells. We demonstrate that the ability of SRF(-/-) embryonic stem (ES) cells to differentiate in vitro into mesodermal cells is indeed impaired. However, this impairment can be modulated by external, cell-independent factors. Retinoic acid, but not dimethylsulfoxide, permitted activation of the mesodermal marker gene T(Bra), which was also activated when SRF was expressed in SRF(-/-) ES cells. Embryoid bodies from SRF(-/-) ES cell aggregates also activated mesodermal marker genes, but displayed unusual morphologies and impairment in cavitation. Finally, in nude mice, Srf(-/-) ES cells readily differentiated into mesodermal cells of SRF(-/-) genotype, including cartilage, bone or muscle cells. We demonstrate that SRF contributes to mesodermal gene expression of ES cells and that SRF(-/-) ES cells display a non-cell-autonomous defect in differentiation towards mesoderm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.