ArticleJournal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
Prevention of mucosal atrophy: role of glutamine and caspases in apoptosis in intestinal epithelial cells.
Article in Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect againstAntioxidants (Basel, Switzerland) · 2021Article
- Impacts of increasing challenge with Eimeria maxima on the growth performance and gene expression of biomarkers associated with intestinal integrity and nutrient transporters.Veterinary research · 2021Article
- Glutaminolysis Epigenetically Regulates Antiapoptotic Gene Expression in Idiopathic Pulmonary Fibrosis Fibroblasts.American journal of respiratory cell and molecular biology · 2019Article
- Glutamine metabolism in advanced age.Nutrition reviews · 2016Review
- Alanyl-glutamine attenuates 5-fluorouracil-induced intestinal mucositis in apolipoprotein E-deficient mice.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- Alanyl-glutamine promotes intestinal epithelial cell homeostasis in vitro and in a murine model of weanling undernutrition.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Proximal gut mucosal epithelial homeostasis in aged IL-1 type I receptor knockout mice after starvation.The Journal of surgical research · 2011Article
- Glutamine supplementation in sick children: is it beneficial?Journal of nutrition and metabolism · 2011Article
- Glutamine protects against apoptosis via downregulation of Sp3 in intestinal epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Alanyl-glutamine and glutamine supplementation improves 5-fluorouracil-induced intestinal epithelium damage in vitro.Digestive diseases and sciences · 2008Article
- Molecular mechanisms contributing to glutamine-mediated intestinal cell survival.American journal of physiology. Gastrointestinal and liver physiology · 2007Article
- Intestinal mucositis: the role of the Bcl-2 family, p53 and caspases in chemotherapy-induced damage.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2006Review
- Clostridium difficile toxin A induces intestinal epithelial cell apoptosis and damage: role of Gln and Ala-Gln in toxin A effects.Digestive diseases and sciences · 2005Article
- Starvation-induced proximal gut mucosal atrophy diminished with aging.JPEN. Journal of parenteral and enteral nutritionArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glutamine starvation induces apoptosis in enterocytes; therefore glutamine is important in the maintenance of gut mucosal homeostasis. However, the molecular mechanisms are unknown. The caspase family of proteases constitutes the molecular machinery that drives apoptosis. Caspases are selectively activated in a stimulus-specific and tissue-specific fashion. The aims of this study were to (1) identify specific caspases activated by glutamine starvation and (2) determine whether a general caspase inhibitor blocks glutamine starvation-induced apoptosis in intestinal epithelial cells. Rat intestinal epithelial (RIE-1) cells were deprived of glutamine. Specific caspase activation was measured using fluorogenic substrate assay. Apoptosis was quantified by DNA fragmentation and Hoechst nuclear staining. Glutamine starvation of RIE-1 cells resulted in the time-dependent activation of caspases 3 (10 hours) and 2 (18 hours), and the induction of DNA fragmentation (12 hours). Caspases 1 and 8 remained inactive ZVAD-fluoromethyl ketone, a general caspase inhibitor, completely blocked glutamine starvation-induced caspase activation, DNA fragmentation, and nuclear condensation. These results indicate that glutamine starvation selectively activates specific caspases, which leads to the induction of apoptosis in RIE-1 cells. Furthermore, inhibition of caspase activity blocked the induction of apoptosis, suggesting that caspases are potential molecular targets to attenuate apoptotic responses in the gut.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.