ArticleProceedings of the National Academy of Sciences of the United States of America2000
RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- Epithelial cells captured from ductal carcinoma in situ reveal a gene expression signature associated with progression to invasive breast cancer.Oncotarget · 2016Article
- Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma.BMC complementary and alternative medicine · 2016Article
- Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction.Cancer research · 2014Article
- AEG-1/MTDH/LYRIC, the beginning: initial cloning, structure, expression profile, and regulation of expression.Advances in cancer research · 2013Review
- Nucleoplasmic calcium regulates cell proliferation through legumain.Journal of hepatology · 2011Article
- Dehydrogenase GRD1 represents a novel component of the cellulase regulon in Trichoderma reesei (Hypocrea jecorina).Applied and environmental microbiology · 2011Article
- Differentially expressed genes in giant cell tumor of bone.Virchows Archiv : an international journal of pathology · 2011Article
- Functional identification of neuroprotective molecules.PloS one · 2010Article
- Genomics and proteomics approaches to the study of cancer-stroma interactions.BMC medical genomics · 2010Article
- Epigenetic silencing of CRABP2 and MX1 in head and neck tumors.Neoplasia (New York, N.Y.) · 2009Article
- Functions of the cytoplasmic RNA sensors RIG-I and MDA-5: key regulators of innate immunity.Pharmacology & therapeutics · 2009Review
- Identification of potential marker genes for Trichoderma harzianum strains with high antagonistic potential against Rhizoctonia solani by a rapid subtraction hybridization approach.Current genetics · 2009Article
- Cloning and characterization of SARI (suppressor of AP-1, regulated by IFN).Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Impact of light on Hypocrea jecorina and the multiple cellular roles of ENVOY in this process.BMC genomics · 2007Article
- Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24): novel gene therapeutic for metastatic melanoma.Toxicology and applied pharmacology · 2007Review
- Profiling Schistosoma mansoni development using serial analysis of gene expression (SAGE).Experimental parasitology · 2007Article
- Astrocyte elevated gene-1: recent insights into a novel gene involved in tumor progression, metastasis and neurodegeneration.Pharmacology & therapeutics · 2007Review
- mda-7/IL-24: multifunctional cancer-specific apoptosis-inducing cytokine.Pharmacology & therapeutics · 2006Review
- Envoy, a PAS/LOV domain protein of Hypocrea jecorina (Anamorph Trichoderma reesei), modulates cellulase gene transcription in response to light.Eukaryotic cell · 2005Article
- ooc1, a unique gene expressed only during growth of Hypocrea jecorina (anamorph: Trichoderma reesei) on cellulose.Current genetics · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Human melanoma cells growth-arrest irreversibly and terminally differentiate on treatment with a combination of fibroblast interferon and the protein kinase C activator mezerein. This experimental protocol also results in a loss of tumorigenic potential and profound changes in gene expression. Various cloning and cDNA microarray strategies are being used to determine the complete spectrum of gene expression changes underlying these alterations in human melanoma cells. An efficient approach, Rapid Subtraction Hybridization (RaSH), has been developed that is permitting the identification of genes of potential relevance to cancer growth control and terminal cell differentiation. RaSH cDNA libraries are prepared from double-stranded cDNAs that are enzymatically digested into small fragments, ligated to adapters, and PCR amplified followed by incubation of tester and driver PCR fragments. This subtraction hybridization scheme is technically simple and results in the identification of a high proportion of differentially expressed sequences, including known genes and those not described in current DNA databases. The RaSH approach represents an efficient methodology for identifying and cloning genes displaying differential expression that associate with and potentially regulate complex biological processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.