ArticleNucleic acids research2000
Characterization of the interaction between alphaCP(2) and the 3'-untranslated region of collagen alpha1(I) mRNA.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Sensing nucleotide composition in virus RNA.Bioscience reports · 2023Review
- Silencing PCBP2 normalizes desmoplastic stroma and improves the antitumor activity of chemotherapy in pancreatic cancer.Theranostics · 2021Article
- Silencing of α-complex protein-2 reverses alcohol- and cytokine-induced fibrogenesis in hepatic stellate cells.Liver research (Beijing, China) · 2017Article
- Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen.Scientific reports · 2016Article
- Regulation of polysome assembly on the endoplasmic reticulum by a coiled-coil protein, p180.Nucleic acids research · 2012Article
- A novel role of RNA helicase A in regulation of translation of type I collagen mRNAs.RNA (New York, N.Y.) · 2012Article
- PCBP2 siRNA reverses the alcohol-induced pro-fibrogenic effects in hepatic stellate cells.Pharmaceutical research · 2011Article
- A novel role of vimentin filaments: binding and stabilization of collagen mRNAs.Molecular and cellular biology · 2011Article
- Targeting collagen expression in alcoholic liver disease.World journal of gastroenterology · 2011Review
- Nonmuscle myosin-dependent synthesis of type I collagen.Journal of molecular biology · 2010Article
- Binding of LARP6 to the conserved 5' stem-loop regulates translation of mRNAs encoding type I collagen.Journal of molecular biology · 2010Article
- Ethanol and arachidonic acid synergize to activate Kupffer cells and modulate the fibrogenic response via tumor necrosis factor alpha, reduced glutathione, and transforming growth factor beta-dependent mechanisms.Hepatology (Baltimore, Md.) · 2008Article
- Structure of a construct of a human poly(C)-binding protein containing the first and second KH domains reveals insights into its regulatory mechanisms.The Journal of biological chemistry · 2008Article
- Cellular protein modification by poliovirus: the two faces of poly(rC)-binding protein.Journal of virology · 2007Article
- RNA-binding protein RBMS3 is expressed in activated hepatic stellate cells and liver fibrosis and increases expression of transcription factor Prx1.Journal of molecular biology · 2007Article
- Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution.Nucleic acids research · 2007Article
- Multimerization of poly(rC) binding protein 2 is required for translation initiation mediated by a viral IRES.RNA (New York, N.Y.) · 2004Article
- SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP.Nucleic acids research · 2004Article
- Leptin induces increased alpha2(I) collagen gene expression in cultured rat hepatic stellate cells.Journal of cellular biochemistry · 2003Article
- Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activated hepatic stellate cells produce increased type I collagen in hepatic fibrosis. The increase in type I collagen protein results from an increase in mRNA levels that is mainly mediated by increased mRNA stability. Protein-RNA interactions in the 3'-UTR of the collagen alpha1(I) mRNA correlate with stabilization of the mRNA during hepatic stellate cell activation. A component of the binding complex is alphaCP(2). Recombinant alphaCP(2) is sufficient for binding to the 3'-UTR of collagen alpha1(I). To characterize the binding affinity of and specificity for alphaCP(2), we performed electrophoretic mobility shift assays using the poly(C)-rich sequence in the 3'-UTR of collagen alpha1(I) as probe. The binding affinity of alphaCP(2) for the 3'-UTR sequence is approximately 2 nM in vitro and the wild-type 3' sequence binds with high specificity. Furthermore, we demonstrate a system for detecting protein-nucleotide interactions that is suitable for high throughput assays using molecular beacons. Molecular beacons, developed for DNA-DNA hybridization, are oligonucleotides with a fluorophore and quencher brought together by a hairpin sequence. Fluorescence increases when the hairpin is disrupted by binding to an antisense sequence or interaction with a protein. Molecular beacons displayed a similar high affinity for binding to recombinant alphaCP(2) to the wild-type 3' sequence, although the kinetics of binding were slower.
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