ArticleNucleic acids research2000
A family of developmentally excised DNA elements in Tetrahymena is under selective pressure to maintain an open reading frame encoding an integrase-like protein.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 19 citations in OpenAlex.
- Structural and Evolutionary Analysis ofInternational journal of molecular sciences · 2025Article
- The polinton-like supergroup of viruses: evolution, molecular biology, and taxonomy.Microbiology and molecular biology reviews : MMBR · 2024Review
- Characterization of Simple Sequence Repeats (SSRs) in Ciliated Protists Inferred by Comparative Genomics.Microorganisms · 2020Article
- RNAi-dependentGenes & development · 2019Article
- Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote.PLoS biology · 2006Article
- Homing endonucleases encoded by germ line-limited genes in Tetrahymena thermophila have APETELA2 DNA binding domains.Eukaryotic cell · 2004Article
- A non-long terminal repeat retrotransposon family is restricted to the germ line micronucleus of the ciliated protozoan Tetrahymena thermophila.Eukaryotic cell · 2004Article
- Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.Eukaryotic cell · 2003Article
- A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophila.Nucleic acids research · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tlr1 is a member of a family of approximately 20-30 DNA elements that undergo developmentally regulated excision during formation of the macronucleus in the ciliated protozoan TETRAHYMENA: Analysis of sequence internal to the right boundary of Tlr1 revealed the presence of a 2 kb open reading frame (ORF) encoding a deduced protein with similarity to retrotransposon integrases. The ORFs of five unique clones were sequenced. The ORFs have 98% sequence conservation and align without frameshifts, although one has an additional trinucleotide at codon 561. Nucleotide changes among the five clones are highly non-random with respect to the position in the codon and 93% of the nucleotide changes among the five clones encode identical or similar amino acids, suggesting that the ORF has evolved under selective pressure to preserve a functional protein. Nineteen T/C transitions in T/CAA and T/CAG codons suggest selection has occurred in the context of the TETRAHYMENA: genome, where TAA and TAG encode Gln. Similarities between the ORF and those encoding retrotransposon integrases suggest that the Tlr family of elements may encode a polynucleotide transferase. Possible roles for the protein in transposition of the elements within the micronuclear genome and/or their developmentally regulated excision from the macronucleus are discussed.
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