ArticleApplied and environmental microbiology2000
Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the chromosome.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Comparative genomics reveals probable adaptations for xylose use in Thermoanaerobacterium saccharolyticum.Extremophiles : life under extreme conditions · 2024Article
- Bacterial xylan utilization regulons: systems for coupling depolymerization of methylglucuronoxylans with assimilation and metabolism.Journal of industrial microbiology & biotechnology · 2022Article
- Article
- Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions.AMB Express · 2017Article
- Genome-scale resources for Thermoanaerobacterium saccharolyticum.BMC systems biology · 2015Article
- Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum.Biotechnology for biofuels · 2013Article
- Metabolic networks evolve towards states of maximum entropy production.Metabolic engineering · 2011Article
- Genetic tool development underpins recent advances in thermophilic whole-cell biocatalysts.Microbial biotechnology · 2011Review
- Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen.Applied and environmental microbiology · 2011Article
- The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass.International journal of biological sciences · 2009Review
- Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Potential and utilization of thermophiles and thermostable enzymes in biorefining.Microbial cell factories · 2007Article
- Electrotransformation of Clostridium thermocellum.Applied and environmental microbiology · 2004Article
- Functional expression of enterobacterial O-polysaccharide biosynthesis enzymes in Bacillus subtilis.Applied and environmental microbiology · 2002Article
- Microbial cellulose utilization: fundamentals and biotechnology.Microbiology and molecular biology reviews : MMBR · 2002Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite recent success in transforming various thermophilic gram-type-positive anaerobes with plasmid DNA, use of shuttle vectors for the expression of genes other than antibiotic resistance markers has not previously been described. We constructed new vectors in order to express heterologous hydrolytic enzymes in our model system, Thermoanaerobacterium saccharolyticum JW/SL-YS485. Transformed Thermoanaerobacterium expressed active enzyme, indicating that this system may function as an alternate expression host, especially for genes with a thermophilic origin. To develop further the genetic system for T. saccharolyticum JW/SL-YS485, two improved Escherichia coli-Thermoanaerobacterium shuttle vectors, pRKM1 and pRUKM, were constructed. Furthermore, the kanamycin resistance cassette alone and the kanamycin resistance cassette plus the cellobiohydrolase gene (cbhA) from Clostridium thermocellum JW20 were integrated into the xylanase gene (xynA) region of the Thermoanaerobacterium chromosome via homologous recombination using pUC-based suicide vectors pUXK and pUXKC.
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