ArticleApplied and environmental microbiology2000
Isolation and use of a homologous histone H4 promoter and a ribosomal DNA region in a transformation vector for the oil-producing fungus Mortierella alpina.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Comparison of Fungal Genera Isolated from Cucumber Plants and Rhizosphere Soil by Using Various Cultural Media.Journal of fungi (Basel, Switzerland) · 2023Article
- Focus and Insights into the Synthetic Biology-Mediated Chassis of Economically Important Fungi for the Production of High-Value Metabolites.Microorganisms · 2023Review
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina.World journal of microbiology & biotechnology · 2021Review
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenases in NADPH Supply in the Oleaginous Filamentous FungusFrontiers in microbiology · 2020Article
- Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.Nature communications · 2018Article
- Enhancing oil production and harvest by combining the marine algaBiotechnology for biofuels · 2018Article
- Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina.BMC biotechnology · 2017Article
- Selection and characterization of promoters based on genomic approach for the molecular breeding of oleaginous fungus Mortierella alpina 1S-4.Current genetics · 2014Article
- Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina.Applied and environmental microbiology · 2014Article
- Versatile transformation system that is applicable to both multiple transgene expression and gene targeting for Thraustochytrids.Applied and environmental microbiology · 2012Article
- Agrobacterium rhizogenes-mediated transformation of a high oil-producing filamentous fungus Umbelopsis isabellina.Journal of applied genetics · 2010Article
- Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding.Applied and environmental microbiology · 2009Article
- Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase.Current genetics · 2009Article
- Disruption of the fatty acid Delta6-desaturase gene in the oil-producing fungus Mortierella isabellina by homologous recombination.Current microbiology · 2007Article
- Article
- A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes.Molecular genetics and genomics : MGG · 2004Article
- Development of a system for integrative and stable transformation of the zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer.Molecular genetics and genomics : MGG · 2004Article
- Identification and characterization of the genes encoding the core histones and histone variants of Neurospora crassa.Genetics · 2002Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mortierella alpina was transformed successfully to hygromycin B resistance by using a homologous histone H4 promoter to drive gene expression and a homologous ribosomal DNA region to promote chromosomal integration. This is the first description of transformation in this commercially important oleaginous organism. Two pairs of histone H3 and H4 genes were isolated from this fungus. Each pair consisted of one histone H3 gene and one histone H4 gene, transcribed divergently from an intergenic promoter region. The pairs of encoded histone H3 or H4 proteins were identical in amino acid sequence. At the DNA level, each histone H3 or H4 open reading frame showed 97 to 99% identity to its counterpart but the noncoding regions had little sequence identity. Unlike the histone genes from other filamentous fungi, all four M. alpina genes lacked introns. During normal vegetative growth, transcripts from the two histone H4 genes were produced at approximately the same level, indicating that either histone H4 promoter could be used in transformation vectors. The generation of stable, hygromycin B-resistant transformants required the incorporation of a homologous ribosomal DNA region into the transformation vector to promote chromosomal integration.
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