ArticleBritish journal of pharmacology2000
5-HT(4) receptors on cholinergic nerves involved in contractility of canine and human large intestine longitudinal muscle.
Article in British journal of pharmacology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 99 citations in OpenAlex.
- The impact of long-course chemoradiotherapy on the myenteric plexus, neuromuscular functions and responses to prokinetic drugs in the human rectum.United European gastroenterology journal · 2024Article
- Preclinical experimental models for assessing laxative activities of substances/products under investigation: a scoping review of the literature.American journal of translational research · 2022Article
- Intraoperative serosal extracellular mapping of the human distal colon: a feasibility study.Biomedical engineering online · 2021Article
- The 5-HT4 Receptor Agonist Prucalopride Stimulates Mucosal Growth and Enhances Carbohydrate Absorption in the Ileum of the Mouse.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2019Article
- cAMP Catalyzing Phosphodiesterases Control Cholinergic Muscular Activity But Their Inhibition Does Not Enhance 5-HTFrontiers in pharmacology · 2018Article
- Effects of Hwangryunhaedok-tang on gastrointestinal motility function in mice.World journal of gastroenterology · 2017Article
- Redundant Gs-coupled serotonin receptors regulate amyloid-β metabolism in vivo.Molecular neurodegeneration · 2016Article
- Distribution of 5-HT3, 5-HT4, and 5-HT7 Receptors Along the Human Colon.Journal of neurogastroenterology and motility · 2015Article
- Serotonin mediation of early memory formation via 5-HT2B receptor-induced glycogenolysis in the day-old chick.Frontiers in pharmacology · 2014Article
- Cholinergic interactions between donepezil and prucalopride in human colon: potential to treat severe intestinal dysmotility.British journal of pharmacology · 2013Article
- Review
- Effects of 5-HT2B, 5-HT3 and 5-HT4 receptor antagonists on gastrointestinal motor activity in dogs.World journal of gastroenterology · 2013Article
- Predominant mucosal expression of 5-HT₄(+h) receptor splice variants in pig stomach and colon.World journal of gastroenterology · 2013Article
- Translational neuropharmacology: the use of human isolated gastrointestinal tissues.British journal of pharmacology · 2013Review
- Anti-diarrheal activity and toxicity of Learng Pid Samud recipe.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2012Article
- Article
- The effects of daikenchuto (DKT) on propulsive motility in the colon.The Journal of surgical research · 2010Article
- Role of prucalopride, a serotonin (5-HT(4)) receptor agonist, for the treatment of chronic constipation.Clinical and experimental gastroenterology · 2010Article
- Therapeutic advances in functional gastrointestinal disease: irritable bowel syndrome.Therapeutic advances in gastroenterology · 2009Article
- Selective desensitization of the 5-HT4 receptor-mediated response in pig atrium but not in stomach.British journal of pharmacology · 2009Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5-HT(4) receptors mediate circular muscle relaxation in both human and canine large intestine, but this phenomenon alone can not explain the improvement in colonic motility induced by selective 5-HT(4) receptor agonists in vivo. We set out to characterize 5-HT(4) receptor-mediated effects in longitudinal muscle strips of canine and human large intestine. Electrical field stimulation (EFS) was applied providing submaximal isotonic contractions. L-NOARG (0.1 mM) was continuously present in the organ bath to preclude nitric oxide-induced relaxation to EFS. The selective 5-HT(4) receptor agonist prucalopride (0.3 microM) enhanced EFS-evoked contractions, that were antagonized in both preparations by the selective 5-HT(4) receptor antagonist GR 113808 (0.1 microM). The prucalopride-induced increase was present in canine ascending and descending colon, but absent in rectum. Regional differences in response to prucalopride were not observed in human ascending and sigmoid colon and rectum. Incubation with atropine (1 microM) or tetrodotoxin (0.3 microM) inhibited EFS-induced contractions, which were then unaffected by prucalopride (0.3 microM) in both tissues. In the presence of methysergide (3 microM; both tissues) and granisetron (0.3 microM; only human tissues), 5-HT (0.3 microM) enhanced EFS-induced contractions, an effect that was antagonized by GR 113808 (0.1 microM). In the presence of atropine or tetrodotoxin, EFS-induced contractions were inhibited, leaving 5-HT (0.3 microM) ineffective in both preparations. This study demonstrates for the first time that in human and canine large intestine, 5-HT(4) receptors are located on cholinergic neurones, presumably mediating facilitating release of acetylcholine, resulting in enhanced longitudinal muscle contractility. This study and previous circular muscle strip studies suggest that 5-HT(4) receptor agonism facilitates colonic propulsion via a coordinated combination of inhibition of circumferential resistance and enhancement of longitudinal muscle contractility.
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