ArticleProceedings of the National Academy of Sciences of the United States of America2000
Purification, molecular cloning, and sequence analysis of sucrose-6F-phosphate phosphohydrolase from plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 87 citations in OpenAlex.
- Unlocking the Genetic Blueprint of Erucic Acid Content in Mustard (Brassica napus): A Meta-QTL Exploration.TheScientificWorldJournal · 2026Article
- Article
- Suppressing wheat sucrose phosphate synthase 1-B protects wheat against stripe rust.Journal of advanced research · 2025Article
- Current perspectives on the regulatory mechanisms of sucrose accumulation in sugarcane.Heliyon · 2024Review
- Cyanobacteria as cell factories for the photosynthetic production of sucrose.Frontiers in microbiology · 2023Review
- Characterization of TaSPP-5A gene associated with sucrose content in wheat (Triticum aestivum L.).BMC plant biology · 2022Article
- Structure and Expression Analysis of Sucrose Phosphate Synthase, Sucrose Synthase and Invertase Gene Families inInternational journal of molecular sciences · 2021Article
- Sucrose-phosphate phosphatase from sugarcane reveals an ancestral tandem duplication.BMC plant biology · 2021Article
- Molecular cloning and expression analysis of sucrose phosphate synthase genes in cassava (Manihot esculenta Crantz).Scientific reports · 2020Article
- Functional Features of TREHALOSE-6-PHOSPHATE SYNTHASE1, an Essential Enzyme in Arabidopsis.The Plant cell · 2020Article
- Transcriptomic Analysis of Gibberellin- and Paclobutrazol-Treated Rice Seedlings under Submergence.International journal of molecular sciences · 2017Article
- Nitrogen-Deficiency Stress Induces Protein Expression Differentially in Low-N Tolerant and Low-N Sensitive Maize Genotypes.Frontiers in plant science · 2016Article
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- Pronounced Phenotypic Changes in Transgenic Tobacco Plants Overexpressing Sucrose Synthase May Reveal a Novel Sugar Signaling Pathway.Frontiers in plant science · 2015Article
- Gloeocapsopsis AAB1, an extremely desiccation-tolerant cyanobacterium isolated from the Atacama Desert.Extremophiles : life under extreme conditions · 2014Article
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- Systematic comparison of C3 and C4 plants based on metabolic network analysis.BMC systems biology · 2012Article
- Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development.Journal of experimental botany · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sucrose-6(F)-phosphate phosphohydrolase (SPP; EC ) catalyzes the final step in the pathway of sucrose biosynthesis and is the only enzyme of photosynthetic carbon assimilation for which the gene has not been identified. The enzyme was purified to homogeneity from rice (Oryza sativa L.) leaves and partially sequenced. The rice leaf enzyme is a dimer with a native molecular mass of 100 kDa and a subunit molecular mass of 50 kDa. The enzyme is highly specific for sucrose 6(F)-phosphate with a K(m) of 65 microM and a specific activity of 1250 micromol min(-1) mg(-1) protein. The activity is dependent on Mg(2+) with a remarkably low K(a) of 8-9 microM and is weakly inhibited by sucrose. Three peptides from cleavage of the purified rice SPP with endoproteinase Lys-C showed similarity to the deduced amino acid sequences of three predicted open reading frames (ORF) in the Arabidopsis thaliana genome and one in the genome of the cyanobacterium Synechocystis sp. PCC6803, as well as cDNA clones from Arabidopsis, maize, and other species in the GenBank database of expressed sequence tags. The putative maize SPP cDNA clone contained an ORF encoding a 420-amino acid polypeptide. Heterologous expression in Escherichia coli showed that this cDNA clone encoded a functional SPP enzyme. The 260-amino acid N-terminal catalytic domain of the maize SPP is homologous to the C-terminal region of sucrose-phosphate synthase. A PSI-BLAST search of the GenBank database indicated that the maize SPP is a member of the haloacid dehalogenase hydrolase/phosphatase superfamily.
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