ArticleProceedings of the National Academy of Sciences of the United States of America2000
Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 119 citations in OpenAlex.
- The impact of K-Ras Gly12 mutants on homeostasis and tumorigenesis in the colonic epithelium.Oncogene · 2026Article
- Multi-ancestral origin of intestinal tumors: Impact on growth, progression, and drug efficacy.Cancer reports (Hoboken, N.J.) · 2022Article
- Loss of MMR and TGFBR2 Increases the Susceptibility to Microbiota-Dependent Inflammation-Associated Colon Cancer.Cellular and molecular gastroenterology and hepatology · 2022Article
- Tissue-Specific Oncogenic Activity of KRASCancer discovery · 2019Article
- Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.Advances in neurobiology · 2018Article
- Advancing Intestinal Organoid Technology Toward Regenerative Medicine.Cellular and molecular gastroenterology and hepatology · 2018Review
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- OncogenicGenes & development · 2017Article
- Global protein expression analysis of molecular markers of DS-1-47, a component of implantation-promoting traditional chinese medicine.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2016Article
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- Colon Tumors with the Simultaneous Induction of Driver Mutations in APC, KRAS, and PIK3CA Still Progress through the Adenoma-to-carcinoma Sequence.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Approaches for targeted proteomics and its potential applications in neuroscience.Journal of biosciences · 2015Review
- Nutrient-induced intestinal adaption and its effect in obesity.Physiology & behavior · 2014Review
- Update on small intestinal stem cells.World journal of gastroenterology · 2013Review
- Laser capture microdissection for protein and NanoString RNA analysis.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Regulation of homeostasis and oncogenesis in the intestinal epithelium by Ras.Experimental cell research · 2011Review
- Context-dependent differentiation of multipotential keratin 14-expressing tracheal basal cells.American journal of respiratory cell and molecular biology · 2011Article
- Role of claudin species-specific dynamics in reconstitution and remodeling of the zonula occludens.Molecular biology of the cell · 2011Article
- Spatial organization of intestinal microbiota in the mouse ascending colon.The ISME journal · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Defining molecular interactions that occur at the interface between "normal" and "abnormal" cell populations represents an important but often underexplored aspect of the pathogenesis of diseases with focal origins. Here, we illustrate an approach for conducting such analyses based on mosaic patterns of Cre recombinase expression in the adult mouse intestinal epithelium. Transgenic mice were generated that express Cre in the stem cell niche of crypts located in specified regions of their intestine. Some of these mice were engineered to allow for doxycycline-inducible Cre expression. Recombination in all pedigrees was mosaic: Cre-expressing crypts that supported recombination in all of their active multipotent stem cells were located adjacent to "control" crypts that did not express Cre at detectable levels. Cre-mediated recombination of a floxed LacZ reporter provided direct evidence that adult small-intestinal crypts contain more than one active multipotent stem cell, and that these cells can be retained in both small-intestinal and colonic crypts for at least 80 d. A method was developed to recover epithelial cells from crypts with or without recombination for subsequent gene expression profiling. Stained sections of intestine were used to create electronic image templates to guide laser capture microdissection (LCM) of adjacent frozen sections. This navigated form of LCM overcomes problems with mRNA degradation encountered when cells are marked directly by immunohistochemical methods. Combining Cre-engineered genetic mosaic mice with navigated-LCM will allow biology and pathobiology to be explored at the junction between normal and perturbed cellular cohorts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.