ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
In situ Ca2+ imaging reveals neurotransmitter receptors for glutamate in taste receptor cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 82 citations in OpenAlex.
- Contribution of TRPC3-mediated CaPflugers Archiv : European journal of physiology · 2023Article
- Pharmacology of the Umami Taste Receptor.Handbook of experimental pharmacology · 2022Article
- Sensing Senses: Optical Biosensors to Study Gustation.Sensors (Basel, Switzerland) · 2020Review
- The Role of the Anion in Salt (NaCl) Detection by Mouse Taste Buds.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Mammalian Taste Cells Express Functional Olfactory Receptors.Chemical senses · 2019Article
- Glutamate: Tastant and Neuromodulator in Taste Buds.Advances in nutrition (Bethesda, Md.) · 2016Review
- Microfluidic Platform with In-Chip Electrophoresis Coupled to Mass Spectrometry for Monitoring Neurochemical Release from Nerve Cells.Analytical chemistry · 2016Article
- Intravital microscopic interrogation of peripheral taste sensation.Scientific reports · 2015Article
- A permeability barrier surrounds taste buds in lingual epithelia.American journal of physiology. Cell physiology · 2015Article
- L-Amino Acids Elicit Diverse Response Patterns in Taste Sensory Cells: A Role for Multiple Receptors.PloS one · 2015Article
- A taste for ATP: neurotransmission in taste buds.Frontiers in cellular neuroscience · 2013Review
- Taste buds as peripheral chemosensory processors.Seminars in cell & developmental biology · 2013Review
- Primary culture of mammalian taste epithelium.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Acetylcholine is released from taste cells, enhancing taste signalling.The Journal of physiology · 2012Article
- Isolation and culture of human fungiform taste papillae cells.Journal of visualized experiments : JoVE · 2012Article
- Adenosine enhances sweet taste through A2B receptors in the taste bud.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Tachykinins stimulate a subset of mouse taste cells.PloS one · 2012Article
- Kokumi substances, enhancers of basic tastes, induce responses in calcium-sensing receptor expressing taste cells.PloS one · 2012Article
- Glutamate may be an efferent transmitter that elicits inhibition in mouse taste buds.PloS one · 2012Article
- Responses to apical and basolateral application of glutamate in mouse fungiform taste cells with action potentials.Cellular and molecular neurobiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The neurotransmitters at synapses in taste buds are not yet known with confidence. Here we report a new calcium-imaging technique for taste buds that allowed us to test for the presence of glutamate receptors (GluRs) in living isolated tissue preparations. Taste cells of rat foliate papillae were loaded with calcium green dextran (CaGD). Lingual slices containing CaGD-labeled taste cells were imaged with a scanning confocal microscope and superfused with glutamate (30 micromter to 1 mm), kainate (30 and 100 micrometer), AMPA (30 and 100 micrometer), or NMDA (100 micrometer). Responses were observed in 26% of the cells that were tested with 300 micrometer glutamate. Responses to glutamate were localized to the basal processes and cell bodies, which are synaptic regions of taste cells. Glutamate responses were dose-dependent and were induced by concentrations as low as 30 microm. The non-NMDA receptor antagonists CNQX and GYKI 52466 reversibly blocked responses to glutamate. Kainate, but not AMPA, also elicited Ca(2+) responses. NMDA stimulated increases in [Ca(2+)](i) when the bath medium was modified to optimize for NMDA receptor activation. The subset of cells that responded to glutamate was either NMDA-unresponsive (54%) or NMDA-responsive (46%), suggesting that there are presumably two populations of glutamate-sensitive taste cells-one with NMDA receptors and the other without NMDA receptors. The function of GluRs in taste buds is not yet known, but the data suggest that glutamate is a neurotransmitter there. GluRs in taste cells might be presynaptic autoreceptors or postsynaptic receptors at afferent or efferent synapses.
Indexed as
Identifiers
11050118PMC6772752W1740107533What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.