ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Phorbol esters potentiate evoked and spontaneous release by different presynaptic mechanisms.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
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- Number and relative abundance of synaptic vesicles in functionally distinct priming states determine synaptic strength and short-term plasticity.The Journal of physiology · 2025Article
- Calcium dependence of spontaneous neurotransmitter release.Journal of neuroscience research · 2018Review
- Molecular underpinnings of synaptic vesicle pool heterogeneity.Traffic (Copenhagen, Denmark) · 2015Review
- The antidepressant fluoxetine mobilizes vesicles to the recycling pool of rat hippocampal synapses during high activity.Molecular neurobiology · 2014Article
- Synaptic vesicle pools and dynamics.Cold Spring Harbor perspectives in biology · 2012Review
- The role of non-canonical SNAREs in synaptic vesicle recycling.Cellular logistics · 2012Article
- Differential regulation of spontaneous and evoked neurotransmitter release at central synapses.Current opinion in neurobiology · 2011Review
- Active zones and the readily releasable pool of synaptic vesicles at the neuromuscular junction of the mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Rapid activation of dormant presynaptic terminals by phorbol esters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Synapsin-dependent vesicle recruitment modulated by forskolin, phorbol ester and ca in mouse excitatory hippocampal synapses.Frontiers in synaptic neuroscience · 2010Article
- Dynamic modulation of phasic and asynchronous glutamate release in hippocampal synapses.Journal of neurophysiology · 2010Article
- beta-Phorbol ester-induced enhancement of exocytosis in large mossy fiber boutons of mouse hippocampus.The journal of physiological sciences : JPS · 2009Article
- Improved spatial learning in aged rats by genetic activation of protein kinase C in small groups of hippocampal neurons.Hippocampus · 2009Article
- Impact of protein kinase C activation on epileptiform activity in the hippocampal slice.Epilepsy research · 2008Article
- Phorbol esters modulate spontaneous and Ca2+-evoked transmitter release via acting on both Munc13 and protein kinase C.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Application of an Epac activator enhances neurotransmitter release at excitatory central synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Developmental changes in brain-derived neurotrophic factor-mediated modulations of synaptic activities in the pontine Kölliker-Fuse nucleus of the rat.The Journal of physiology · 2007Article
- Differential control of two forms of glutamate release by group III metabotropic glutamate receptors at rat entorhinal synapses.Neuroscience · 2007Article
- Silencing of ZnT-1 expression enhances heavy metal influx and toxicity.Journal of molecular medicine (Berlin, Germany) · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Phorbol esters enhance release from a variety of cell types. The mechanism by which phorbol esters potentiate presynaptic release from central neurons is unclear, although effects of phorbol esters both on the readily releasable pool of vesicles and on presynaptic calcium channels have been shown. Using confocal microscopy and the fluorescent styryl dye FM 1-43, we have examined the effects of phorbol-12,13-dibutyrate (PDBu) on presynaptic vesicle turnover at individually identified synapses in dissociated cultures obtained from neonatal rat hippocampus. Using different dye staining and destaining protocols we were able to resolve two effects of PDBu. Potentiation of evoked release by PDBu was insensitive to calcium channel antagonists, suggesting that this effect results from an increased number of vesicles in the readily releasable pool. Since we observed no effect of PDBu on the size of the total recycling vesicle pool, we conclude that phorbol esters alter the equilibrium between reserve and readily releasable pools. An additional effect of PDBu on spontaneous release was observed. This effect was antagonized by nifedipine but not omega-conotoxin GVIA or omega-agatoxin IVA. We conclude that PDBu influences spontaneous and evoked release by two different mechanisms: through L-type calcium channels and through an increase in the proportion of recycling vesicles in the readily releasable pool. In addition to further clarifying the mechanism of action of phorbol esters, these results suggest that phorbol esters may be a useful tool with which to probe the function of the readily releasable pool of presynaptic vesicles at CNS synapses.
Indexed as
Identifiers
11050105PMC6772716W1912391091What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.