ArticleMolecular and cellular biology2000
Developmentally regulated excision of a 28-base-pair sequence from the Paramecium genome requires flanking DNA.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Programmed genome rearrangements in ciliates.Cellular and molecular life sciences : CMLS · 2020Review
- The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.Eukaryotic cell · 2010Article
- Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: a somatic view of the germline.Genome research · 2008Article
- PAK paradox: Paramecium appears to have more K(+)-channel genes than humans.Eukaryotic cell · 2003Article
- Control of DNA excision efficiency in Paramecium.Nucleic acids research · 2001Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The micronuclear DNA of Paramecium tetraurelia is estimated to contain over 50,000 short DNA elements that are precisely removed during the formation of the transcriptionally active macronucleus. Each internal eliminated sequence (IES) is bounded by 5'-TA-3' dinucleotide repeats, a feature common to some classes of DNA transposons. We have developed an in vivo assay to analyze these highly efficient and precise DNA excision events. The microinjection of a cloned IES into mating cells results in accurately spliced products, and the transformed cells maintain the injected DNA as extrachromosomal molecules. A series of deletions flanking one side of a 28-bp IES were constructed and analyzed with the in vivo assay. Whereas 72 bp of DNA flanking the eliminated region is sufficient for excision, lengths of 31 and 18 bp result in reduced excision and removal of all wild-type sequences adjacent to the TA results in complete failure of excision. In contrast, nucleotide mutations within the middle of the 28-bp IES do not prevent excision. The results are consistent with a functional role for perfect inverted repeats flanking the IES.
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Registered trials
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