ReviewPhotochemistry and photobiology2000
On spectral relaxation in proteins.
Review in Photochemistry and photobiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Indocyanine green excitation-emission matrix characterization: excitation-dependent emission shifts and application-specific spectra.Journal of biomedical optics · 2026Article
- Indocyanine green excitation-emission matrix characterization: excitation-dependent emission shifts and application-specific spectra.bioRxiv : the preprint server for biology · 2025Article
- Organic-solvent mediated self-assembly of protoporphyrin IX with human serum albumin.International journal of biological macromolecules · 2025Article
- Spectral and conformational characteristics of phycocyanin associated with changes of medium pH.Photosynthesis research · 2024Article
- Identification of Surfactant Impact on a Monoclonal Antibody Characterization via HPLC-Separation Based and Biophysical Methods.Pharmaceutical research · 2024Article
- Advanced Spectroscopy and APBS Modeling for Determination of the Role of His190 and Trp103 in Mouse Thymidylate Synthase Interaction with Selected dUMP Analogues.International journal of molecular sciences · 2021Article
- Developing Analysis Protocols for Monitoring Intracellular Oxygenation Using Fluorescence Lifetime Imaging of Myoglobin-mCherry.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Interface Compositions as Determinants of Resveratrol Stability in Nanoemulsion Delivery Systems.Foods (Basel, Switzerland) · 2020Article
- Tryptophan / Dextran70 Based - Fluorescent Silver Nanoparticles: Synthesis and Physicochemical Properties.Journal of fluorescence · 2019Article
- Conformational Dynamics Govern the Free-Energy Landscape of a Membrane-Interacting Protein.ACS omega · 2018Article
- Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells.American journal of physiology. Cell physiology · 2018Article
- Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches.International journal of environmental research and public health · 2018Article
- Label-free imaging of amyloids using their intrinsic linear and nonlinear optical properties.Biomedical optics express · 2017Article
- Effects of Visible-Light Irradiation of Protoporphyrin IX on the Self-Assembly of Tubulin Heterodimers.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Article
- Wide-field medium-repetition-rate multiphoton microscopy reduces photodamage of living cells.Biomedical optics express · 2016Article
- Picosecond fluorescence dynamics of tryptophan and 5-fluorotryptophan in monellin: slow water-protein relaxation unmasked.The journal of physical chemistry. B · 2015Article
- Exploring protein solution structure: Second moments of fluorescent spectra report heterogeneity of tryptophan rotamers.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2015Article
- Role of the membrane in the formation of heme degradation products in red blood cells.Life sciences · 2010Article
- Excited-state lifetime studies of the three tryptophan residues in the N-lobe of human serum transferrin.Protein science : a publication of the Protein Society · 2010Article
- Femtosecond fluorescence spectra of tryptophan in human gamma-crystallin mutants: site-dependent ultrafast quenching.Journal of the American Chemical Society · 2009Article
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Abstract
During the past several years there has been debate about the origins of nonexponential intensity decays of intrinsic tryptophan (trp) fluorescence of proteins, especially for single tryptophan proteins (STP). In this review we summarize the data from diverse sources suggesting that time-dependent spectral relaxation is a ubiquitous feature of protein fluorescence. For most proteins, the observations from numerous laboratories have shown that for trp residues in proteins (1) the mean decay times increase with increasing observation wavelength; (2) decay associated spectra generally show longer decay times for the longer wavelength components; and (3) collisional quenching of proteins usually results in emission spectral shifts to shorter wavelengths. Additional evidence for spectral relaxation comes from the time-resolved emission spectra that usually shows time-dependent shifts to longer wavelengths. These overall observations are consistent with spectral relaxation in proteins occurring on a subnanosecond timescale. These results suggest that spectral relaxation is a significant if not dominant source of nonexponential decay in STP, and should be considered in any interpretation of nonexponential decay of intrinsic protein fluorescence.
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